等离子体技术产生的羟基自由基对医疗器械灭菌效果探讨  

Discussion on the sterilization effect of hydroxyl radicals produced by plasma technology on medical equipment

作　　者：闵捷[1] 陈捷[2] Min Jie;Chen Jie(Nanjing Pukou District Central Hospital,Nanjing 211800,Jiangsu;The First Affiliated Hospital of Nanjing Medical University(Jiangsu Provincial People's Hospital),Nanjing 210000,Jiangsu)

机构地区：[1]南京市浦口区中心医院,江苏南京211800 [2]南京医科大学第一附属医院(江苏省人民医院),江苏南京210000

出　　处：《现代科学仪器》2020年第6期56-59,共4页Modern Scientific Instruments

摘　　要：本研究以0.5McFarland之大肠杆菌及金黄色葡萄球菌为研究对象,比较血浆溶液(PS)及血浆介电屏障放电(PDBD)两种技术对手术刀之杀菌效果。实验设计采用十倍稀释平板计数法确定了两种方法均能达到100%效果的最佳条件。对于PS,电压设定为520v,时间为20s,醋酸钠浓度为1g/L,对于PDBD,阳极间距为9.4mm,放电时间为14min,功率为76w,氩气流量为22L/min,比较两种方法100%灭菌时间,发现PS使用较少时间比PDBD。然而,经光发射光谱(OES)测试,发现PDBD产生的羟基自由基大于PS产生的羟基自由基。This study examined sterilization of scalpels using plasma technology by comparing the performance effectiveness of two techniques,plasma solution(PS)and plasma dielectric barrier discharge(PDBD),on Escherichia coli and Staphylococcus aureus at 0.5 McFarland.The experimental design was used to determine the optimal condition for both the techniques to be 100%effective by using the ten-fold dilution spread plate count.For PS,the voltage was set at 520 V for 20 s and the concentration of sodium acetate was 1 g/L.For PDBD,the distance between the anodes was 9.4 mm;the discharge time was 14 min;the power was 76 W;and the argon gas flow rate was 22 L/min.On comparing the time taken for 100%sterilization by the two techniques,it was found that PS used less time than PDBD.However,upon testing with Optical Emission Spectrometry(OES),the hydroxyl free radicals produced by PDBD were observed to be greater than those produced by PS.

关 键 词：血浆 PDBD 大肠杆菌 金黄色葡萄球菌 OES 

分 类 号：R28[医药卫生—中药学]