口服金霉素微囊化颗粒对绵羊瘤胃微生物菌群数量的影响  

Effect of orally administrated chlortetracycline microcapsule on microflora quantity in sheep rumen

作　　者：李国基 胡浪 李荣顺 李大彪[3] 杨硕[3] 邢媛媛[3] 黄显会[1] LI Guoji;HU Lang;LI Rongshun;LI Dabiao;YANG Shuo;XING Yuanyuan;HUANG Xianhui(College of Veterinary Medicine,South China Agricultural University/Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation,Guangzhou 510642,China;Jinhe Biotechnology Co.Ltd.,Hohhot 010018,China;College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]华南农业大学兽医学院/广东省兽药研制与安全评价重点实验室,广东广州510642 [2]金河生物科技股份有限公司,内蒙古呼和浩特010018 [3]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018

出　　处：《华南农业大学学报》2021年第1期17-25,共9页Journal of South China Agricultural University

基　　金：国家科技支撑计划(2015BAD11B03-06)。

摘　　要：【目的】评估口服自主研制的金霉素微囊化颗粒对绵羊瘤胃微生物菌群数量的影响。【方法】选取体质量(29.0±2.3)kg、6月龄左右、体况良好的18只绵羊,随机分为6组,包括1个空白对照组和5个给药组,每组3只。空白对照组只饲喂基础饲粮;5个给药组分别为试验I组:基础饲粮+普通金霉素预混剂颗粒,试验II组:基础饲粮+氢化油处方组金霉素微囊化颗粒,试验III组:基础饲粮+PEG4000/单甘脂(质量比为5∶5)处方组金霉素微囊化颗粒,试验IV组:基础饲粮+氢化油/单甘脂(质量比为9∶1)处方组金霉素微囊化颗粒,试验V组:基础饲粮+氢化油/单甘脂(质量比为8∶2)处方组金霉素微囊化颗粒。给药组绵羊金霉素最终给药剂量均为25 mg/kg,每天给药1次且均在晨饲前灌胃给药,连续给药5 d,通过瘤胃液采样器采集瘤胃内容物。采集的瘤胃内容物经4层纱布过滤后,收集滤液装于冻存管内,所有样品经液氮速冻后置于−80℃保存,运用实时荧光定量PCR(RT-PCR)技术对绵羊瘤胃液相瘤胃微生物进行定量检测。【结果】与普通金霉素预混剂颗粒相比,氢化油/单甘脂(质量比为9∶1)处方组金霉素微囊化颗粒有较好效果,只显著增加了产琥珀酸拟杆菌的数量,对绵羊瘤胃其他微生物菌群数量影响均不显著;与空白对照组相比,氢化油/单甘脂(质量比为9∶1)处方组金霉素微囊化颗粒及PEG4000/单甘脂(质量比为5∶5)处方组金霉素微囊化颗粒有相对较好效果,分别只对牛链球菌及产琥珀酸拟杆菌的数量有显著降低趋势,对绵羊瘤胃其他微生物菌群数量影响均不显著。【结论】氢化油/单甘脂(质量比为9︰1)处方组金霉素微囊化颗粒对绵羊瘤胃微生物数量抑制作用较小,符合临床应用要求。【Objective】To evaluate the effect of orally administrated self-developed chlortetracycline microcapsule on microflora quantity in sheep rumen.【Method】Eighteen sheep of(29.0±2.3)kg of 6-month-old with good health condition were randomly assigned to six groups with three replicates in eash grcup,including one control group and five medicated groups.The sheep in the control group were only fed with basal diet.The treatments in five medicated groups were as follows.Group I:Basal diet supplemented with normal chlortetracycline premix granule;Group II:Basal diet supplemented with hydrogenated oil prescription chlortetracycline microcapsule;Group III:Basal diet supplemented with PEG4000/glycerol stearate(mass ratio of 5︰5)prescription chlortetracycline microcapsule;Group IV:Basal diet supplemented with hydrogenated oil/glycerol stearate(mass ratio of 9︰1)prescription chlortetracycline microcapsule;Group V:Basal diet supplemented with hydrogenated oil/glycerol stearate(mass ratio of 8︰2)prescription chlortetracycline microcapsule.The chlortetracycline administration dosages of sheep in the five medicated groups were all 25 mg/kg.The sheep were administrated once a day before morning feeding for five days.The rumen contents were collected by rumen fluid collector and quickly filtered through four layers of gauze after continuous administration.The filtrate were collected in the cryopreserved tube and quickly frozened by liquid nitrogen then stored at−80℃.The rumen microorganisms of liquid phase were quantitatively detected by real-time fluorescence quantitative PCR(RT-PCR).【Result】Compared with the normal chlortetracycline premix granule,hydrogenated oil/glycerol stearate(mass ratio of 9︰1)prescription chlortetracycline microcapsule had better effect,only significantly increased the quantity of Fibrobacter succinogens,and had no significant influence on the quantities of other microbial flora in sheep rumen.Compared with the control group,hydrogenated oil/glycerol stearate(mass ratio of 9︰1)pres

关 键 词：绵羊 瘤胃 微生物 金霉素预混剂 金霉素微囊化颗粒 

分 类 号：S826[农业科学—畜牧学]