HPLC法同时测定覆盆子中7个成分的含量  

Simultaneous Determination of Seven Constituents in Rubus Chingii by HPLC

作　　者：张晶[1] 汤尘尘[1] 方艳[1] Zhang Jing;Tang Chenchen;Fang Yan(Pharmacy Department of Hunan Children's Hospital,Changsha 410007,China)

机构地区：[1]湖南省儿童医院药学部,长沙410007

出　　处：《中国药师》2020年第12期2496-2499,共4页China Pharmacist

摘　　要：目的:建立同时测定覆盆子中鞣花酸、山柰酚-3-O-芸香糖苷、椴树苷、山柰酚、槲皮素、芦丁和齐墩果酸含量的方法。方法:采用HPLC色谱法,色谱柱为Thermo ODS C18(250 mm×4.6 mm,5μm),以乙腈-0.2%磷酸水溶液为流动相,梯度洗脱,流速:1.0 ml·min-1,检测波长:254 nm(鞣花酸、槲皮素、山柰酚)、340 nm(山柰酚-3-O-芸香糖苷、芦丁)、360 nm(齐墩果酸)、316 nm(椴树苷),柱温:30℃,进样量:20μl。结果:鞣花酸、山柰酚-3-O-芸香糖苷、椴树苷、山柰酚、槲皮素、芦丁和齐墩果酸检测质量浓度的线性范围分别为14.412~172.944μg·ml-1,5.042~60.504μg·ml-1,0.605~7.260μg·ml-1,1.151~13.812μg·ml-1,2.562~30.744μg·ml-1,4.451~53.412μg·ml-1,0.507~6.084μg·ml-1(r为0.9994~0.9999);平均加样回收率分别为101.3%,99.6%,100.5%,100.8%,101.0%,99.7%,100.3%(RSD<2.0%,n=6)。结论:该方法操作简便、结果准确、重复性好,可用于覆盆子的质量控制。Objective:To develop a method for the simultaneous determination of ellagic acid,kaempferol-3-O-rutinoside,tiliroside,kaempferol,quercetin,rutin and oleanolic acid in Rubus chingii by HPLC.Methods:The analysis was carried out on a Thermo ODS C18column(250 mm×4.6 mm,5μm) with the mobile phase consisting of acetonitrile-0.2%phosphoric acid with a gradient elution mode at a flow rate of 1.0 ml·min-1.The detection wavelength was set at 254 nm for ellagic acid,quercetin and kaempferol,340 nm for kaempferol-3-O-rutinoside and rutin,360 nm for oleanolic acid,and 316 nm for tiliroside.The column temperature was set at 30℃and the injection volume was 20μl.Results:The linear range was 14.412-172.944μg·ml-1,5.042-60.504μg·ml-1,0.605-7.260μg·ml-1,1.151-13.812μg·ml-1,2.562-30.744μg·ml-1,4.451-53.412μg·ml-1and 0.507-6.084μg·ml-1(r=0.999 4-0.999 9) for ellagic acid,rutin,kaempferol-3-O-rutinoside,oleanolic acid,tiliroside,quercetin and kaempferol,respectively (r=0.999 4-0.999 9).The average recovery was 101.3%,99.6%,100.5%,100.8%,101.0%,99.7%and 100.3%(RSD<2.0%,n=6),respectively.Conclusion:The established method is simple,accurate and reproducible,which can be used for the simultaneous determination of seven active constituents in Rubus chingii.

关 键 词：覆盆子 鞣花酸 山柰酚-3-O-芸香糖苷 椴树苷 山柰酚 槲皮素 芦丁 齐墩果酸 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]