一株絮凝剂产生菌死谷芽孢杆菌Z11的筛选和鉴定  

Screening and identification of a flocculant-producing strain Bacillus vallismortis Z11

作　　者：张晓飞 孙云龙 韩国红 李刻秦 师晓喆 胡加超 董淑敏 王铮 赵哲 于茹 曲杰 宋峰 李正华 ZHANG Xiaofei;SUN Yunlong;HAN Guohong;LI Keqin;SHI Xiaozhe;HU Jiachao;DONG Shumin;WANG Zheng;ZHAO Zhe;YU Ru;QU Jie;SONG feng;LI Zhenghua(Shandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, Shandong 253023, China;College of Life Sciences, Dezhou University, Dezhou, Shandong 253023, China;Feicheng Number Three Middle School, Taian, Shandong 271600, China)

机构地区：[1]山东省生物物理重点实验室,山东德州253023 [2]德州学院生命科学学院,山东德州253023 [3]肥城市第三高级中学,山东泰安217600

出　　处：《工业微生物》2020年第6期18-24,共7页Industrial Microbiology

基　　金：国家自然科学基金青年基金(31800106);山东省自然科学基金博士基金项目(ZR2019BC027);山东省农业微生物重点实验室开放课题基金(SDKL2017015)。

摘　　要：从德州卓奥水质净化有限公司的活性污泥中分离筛选出具有良好絮凝效果的菌株,并通过优化培养条件,研究其絮凝特性。通过稀释涂布和平板划线法筛选得到7株具有较强絮凝活性的菌株,对絮凝效果最好的菌株Z11进行形态、生理生化特性以及16S rRNA序列分析,初步鉴定其种属;并采用单因素实验及高岭土悬浊液絮凝沉降试验,优化菌株的培养条件,并初步研究菌株Z11的絮凝特性。初步鉴定菌株Z11为死谷芽孢杆菌(Bacillus vallismortis),在菌株培养液添加量3 mL、pH 5.0、温度30℃、转速220 r/min条件下,对4 g/mL高岭土悬浊液和24 g/L 1%氯化钙溶液混合液的絮凝率最高为96.66%。死谷芽孢杆菌Z11表现出良好的絮凝效果,具有应用污水处理的巨大潜力,有望应用于高效率的污水处理。Strains with good flocculant effects were isolated from the activated sludge of Zhuo Ao water purification Co.,Ltd in Dezhou and their flocculation characteristics were investigated by optimizing culture conditions.Seven strains with strong flocculation activity were screened by dilution and spread plate method and plate streaking method.A flocculat-producing strain Z11 was obtained with the best flocculating effect.The single factor experiment and Kaolin suspension flocculation sedimentation experiment were used to optimize the culture conditions,and the flocculation characteristics of strain Z11 were preliminarily studied.The strain Z11 was tentatively identified as Bacillus vallismortis based on its morphological and physiological-biochemical characteristics and 16S rRNA sequence analysis.The optimum culture conditions for Z11 were determined as follows:MBF dosage of 0.0048 g/L,pH 5,temperature 30℃,rate of shaking incubator 220 r/min,Kaolin concentration 4 g/mL,using 24 g/L of CaCl(mass concentration of 1%)as coagulant.Under the optimized conditions,the flocculating rate of Z11 reached as high as 96.66%.The results revealed that the Bacillus vallismortis Z11 showed good flocculation effect and had great potential for efficiency sewage treatment.

关 键 词：絮凝沉降法 微生物絮凝剂(MBF) 死谷芽孢杆菌Z11 

分 类 号：X70[环境科学与工程—环境工程]