无油安全套对精子受精功能与DNA碎片指数检测结果影响的观察  

Effects of oil-free condoms on sperm fertilization function and DFI

作　　者：赵晋 黄泗翀 黄立光[1] 邓泽桦 关艺青 张汉奎[1] ZHAO Jin;HUANG Sichong;HUANG Liguang;DENG Zehua;GUAN Yiqing;ZHANG Hankui(Zhongshan City People’s Hospital, Guangdong Province, 528400)

机构地区：[1]广东省中山市人民医院,528400

出　　处：《中国计划生育学杂志》2020年第11期1768-1770,共3页Chinese Journal of Family Planning

摘　　要：目的:观察无油安全套对精子的受精功能和精子DNA碎片指数(DFI)检测结果的影响。方法:实验组在无油安全套中加入1.0ml液化精液,置于振荡器轻柔振荡5min后取出,转移至5ml离心管,15min后检测精子活力,随后立即检测精子顶体酶活性、精子顶体反应率及DFI,并与未与无油安全套接触的对照组精液进行比较。结果:实验组精子活力(58.7±12.4)%,低于对照组(63.6±11.4)%(P<0.05);顶体酶活性(102.0±26.3)μU/106高于精子较对照组(84.6±23.0)μU/106(P<0.05);顶体反应率(40.7±14.8)%低于对照组(48.4±15.8)%(P<0.01);DFI结果两组无差异(P>0.05)。结论:进行精子活力、精子顶体酶活性及精子顶体反应检测时不建议使用无油安全套收集标本。Objective:To observe the effect of oil-free condom on sperm fertilization function and the detection results of DNA fragment index(DFI)of sperm.Methods:1.0ml liquefied semen was puted in the oil-free condom in the experimental group,which was placed in an oscillator and gently oscillated for 5min before being removed,and then was transferred to a 5ml centrifugation tube.1.0ml liquefied semen was not in oil-free condom in the control group.Sperm motility in both groups was detected 15 min later,and then sperm acrosin activity,acrosomal reaction rate and DFI were immediately detected.The semen was compared between the experimental group and the control group.Results:The sperm motility of the experimental group was 58.7±12.4%was significant lower than that(63.6±11.4%)of the control group(P<0.05).The activity of sperm acrosin of the experimental group was 102.0±26.3 U/106 sperm was significant higher than that(84.6±23.0 U/106 sperm)of the control group(P<0.05).The acrosomal reaction rate of the experimental group was 40.7±14.8%was significant lower than that(48.4±15.8%)of the control group(P<0.01).There was no significant difference in DFI between the two groups(P>0.05).Conclusion:It is not recommended to use oil-free condoms to collect samples for the detection of sperm motility,sperm acrosin activity and sperm acrosome reaction.

关 键 词：安全套 生物毒性 精子 精子顶体酶活性 精子顶体反应 DNA碎片指数 

分 类 号：R69[医药卫生—泌尿科学]