基于UPLC-MS/MS技术研究濒死状态下新生羔羊血清代谢物变化  

Serum Metabolic Analysis of Antemortem Neonatal Goats Using the Approach of UPLC-MS/MS

作　　者：程艳 杨超 许丽卫[1] 耿梅梅[1] 谭支良[1,2] 贺志雄 CHENG Yan;YANG Chao;XU Liwei;GENG Meimei;TAN Zhiliang;HE Zhixiong(Key Laboratory for Agro-Ecological Processes in Subtropical Region,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,畜禽养殖污染控制与资源化技术国家工程实验室,湖南省动物营养生理与代谢过程重点实验室,农业部中南动物营养与饲料科学观测实验站,长沙410125 [2]中国科学院大学,北京100049

出　　处：《畜牧兽医学报》2020年第12期3199-3204,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31772631);中国科学院STS项目(KFJ-STS-ZDTP-075);湖南创新型省份建设专项(2019RS3021)。

摘　　要：基于流行病学的角度,旨在初步探索与新生羔羊疾病相关的代谢标识物。本研究采用病理对照试验设计,利用高效液相色谱串联质谱比较病危临死新生羔羊与健康新生羔羊(相同日龄)的血清代谢差异。结果显示:两组血清代谢物能得到较好区分,共鉴定出13种差异代谢物,KEGG富集分析显示,这些差异代谢物主要富集在氨基酸生物合成和代谢通路上。羔羊濒死过程中血清内源性代谢物发生明显改变,可为新生羔羊疾病诊断标识物的筛选和构建提供基础。This study aimed to explore the metabolic differences in sera of antemortem neonatal goats based on an epidemiological perspective.A case-control design was used to discover the metabolic differences in serum between the dead newborn goats suffered from a disease and healthy goats at the same age by using UPLC-MS/MS.A total of 13 compounds were significantly different between healthy and dead newborn goats.KEGG analysis showed that these differential produced metabolites mainly involved the synthesis and catabolism of amino acids.The differential produced metabolites in serum are important for us to understand the agonal reaction at the metabolic level when neonatal goats suffering from a disease.This study also provides an advanced research basis for the establishment of disease-related biomarkers for neonatal goats which may suffer from a disease.

关 键 词：新生羔羊 代谢组学 高效液相色谱串联质谱 疾病诊断技术 

分 类 号：S857.31[农业科学—临床兽医学]