Insect resistance management in Bacillus thuringiensis cotton by MGPS(multiple genes pyramiding and silencing)  

作　　者：ZAFAR Muhammad Mubashar RAZZAQ Abdul FAROOQ Muhammad Awais REHMAN Abdul FIRDOUS Hina SHAKEEL Amir MO Huijuan REN Maozhi 

机构地区：[1]Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang 455000,Henan,China [2]Department of Plant Breeding and Genetics,University of Agriculture Faisalabad,Faisalabad 38000,Pakistan [3]Zhengzhou Research Base,State Key Laboratory of Cotton Biology,Zhengzhou University,Zhengzhou 450000,China [4]Department of Plant Pathology,University of Agriculture Faisalabad,Faisalabad,Pakistan.

出　　处：《Journal of Cotton Research》2020年第4期311-323,共13页棉花研究（英文）

基　　金：This work was supported by the Genetically Modified Organisms Breeding Major Project of China(2019ZX08010004–004);the National Natural Science Foundation of China(31901579).

摘　　要：The introduction of Bacillus thuringiensis(Bt)cotton has reduced the burden of pests without harming the environment and human health.However,the efficacy of Bt cotton has decreased due to field-evolved resistance in insect pests over time.In this review,we have discussed various factors that facilitate the evolution of resistance in cotton pests.Currently,different strategies like pyramided cotton expressing two or more distinct Bt toxin genes,refuge strategy,releasing of sterile insects,and gene silencing by RNAi are being used to control insect pests.Pyramided cotton has shown resistance against different cotton pests.The multiple genes pyramiding and silencing(MGPS)approach has been proposed for the management of cotton pests.The genome information of cotton pests is necessary for the development of MGPS-based cotton.The expression cassettes against various essential genes involved in defense,detoxification,digestion,and development of cotton pests will successfully obtain favorable agronomic characters for crop protection and production.The MGPS involves the construction of transformable artificial chromosomes,that can express multiple distinct Bt toxins and RNAi to knockdown various essential target genes to control pests.The evolution of resistance in cotton pests will be delayed or blocked by the synergistic action of high dose of Bt toxins and RNAi as well as compliance of refuge requirement.

关 键 词：Bt cotton RNAI Multiple genes pyramiding and silencing(MGPS) Bt resistance 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]