黄喉拟水龟性别调控相关lncRNA和mRNA的筛选及初步分析  

Identification and analysis of lncRNAs and mRNAs involved in sex regulation in Asian yellow pond turtle(Mauremys mutica)

作　　者：朱彦雨 刘晓莉 王亚坤[1] 李伟[1] 洪孝友[1] 朱新平[1,2] 徐红艳 ZHU Yanyu;LIU Xiaoli;WANG Yakun;LI Wei;HONG Xiaoyou;ZHU Xinping;XU Hongyan(Key Laboratory of Tropical&Subtropical Fishery Resource Application&Cultivation of Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]中国水产科学研究院珠江水产研究所,农业农村部热带亚热带水产资源利用与养殖重点实验室,广东广州510380 [2]上海海洋大学,水产科学国家级实验教学示范中心,上海201306

出　　处：《水产学报》2020年第12期1960-1975,共16页Journal of Fisheries of China

基　　金：国家重点研发计划(2018YFD0900201,2018YFD0900203);国家自然科学基金(31873035);广州市科技计划项目(201904010172);社会公益研究(2019HY-XKQ02);社会公益研究(2018SJ-YZ02);中国—东盟海上合作基金资助(CAMC-2018F);“一带一路”沿线国家热带农业科技创新合作(2130114)。

摘　　要：性别异形在动物界广泛存在,长期以来一直是生物学中的热门话题。黄喉拟水龟性别分化属温度依赖型,其温度响应分子机制仍不清楚。为了进一步解析龟鳖动物性别分化的分子机制,本研究初步比较分析了黄喉拟水龟转录本中性别差异表达(different expressed, DE)的长链非编码RNA(long noncoding RNA, lncRNA)及其调控的靶基因。首先,运用Illumina深度测序平台,通过转录组测序(RNA-Seqs)对黄喉拟水龟的精巢和卵巢进行了比较转录组学分析并筛选鉴定出雌雄差异转录本,共筛选获得8 237个DE mRNA和9 573个DE lncRNA。通过GO功能注释及KEGG通路富集分析发现,上述差异转录本主要参与龟鳖动物性别分化和性腺发育等相关信号通路。此外,通过顺式和反式作用分析,筛选获得了一系列与生殖发育相关的(性腺发育和性别分化)受DE lncRNAs调控的靶基因。本研究为进一步阐明黄喉拟水龟温度依赖型性别的分子机制提供了线索,特别是为进一步挖掘利用龟鳖动物性别决定因子,进行龟鳖动物性控育种技术研究奠定了基础。Sexual dimorphism is widespread in animal kingdom and has been a hot topic in biology for a long time.In particular, the sex of the Mauremys mutica is determined by incubation temperature, however, the molecular mechanism of temperature response is still unclear. In order to understand the molecular mechanism of the sex differentiation of turtles, this study made a preliminary comparative analysis of the sex-differentiated lncRNAs and their target genes in the transcripts of M. mutica. First, the Illumina deep sequencing platform was used to perform comparative transcriptomics analysis of the testis and ovary of M. mutica by RNA-Seqs and screened to identify male and female differential transcripts. A total of 8 237 differentially expressed mRNAs and 9 573 differentially expressed lncRNAs were screened. Through GO functional annotation and KEGG pathway enrichment analysis, we found that these differential transcripts were mainly involved in the sex differentiation and gonadal development of turtles. In addition, a series of target genes related to reproductive development(gonad development and sex differentiation) regulated by DE lncRNA were obtained through the analysis of cis-and transeffects. This study provides clues for further elucidating the temperature-dependent sex differentiation mechanism of M. mutica, and especially, it lays the foundation for further exploring the use of the sex determinants of turtles to conduct research on animal sexuality control breeding techniques for turtles.

关 键 词：黄喉拟水龟 转录组 长链非编码RNA 性别决定 性别分化 

分 类 号：Q785[生物学—分子生物学] S917.4[农业科学—水产科学]