肝癌癌组织PCDH8、RIZ1基因甲基化状态及临床意义  

Methylation status and clinical significance of PCDH8 and RIZ1 genes in liver cancer tissues

作　　者：陈素云 赵志新 褚邦勇 CHEN Suyun;ZHAO Zhixin;CHU Bangyong(Department of Clinical Laboratory,Taizhou Hospital,Taizhou Enze Medical Center(Group),Taizhou,Zhejiang 317000,China;Department of Neurosurgery,Taizhou Hospital,Taizhou Enze Medical Center (Group),Taizhou,Zhejiang 317000,China)

机构地区：[1]台州恩泽医疗中心(集团)台州医院检验科,317000 [2]台州恩泽医疗中心(集团)台州医院神经外科,317000

出　　处：《重庆医学》2020年第24期4075-4079,4084,共6页Chongqing medicine

基　　金：浙江省自然科学基金项目(LY19H200001);恩泽医疗中心(集团)科学研究基金(17EZC3)。

摘　　要：目的探讨肝癌癌组织PCDH8、RIZ1基因甲基化状态及其临床意义。方法选取80例肝癌癌组织、癌旁组织及50例正常肝组织作为研究标本,采用甲基化特异性聚合酶链式反应技术检测组织PCDH8、RIZ1基因甲基化状态,分析其与肝癌患者临床病理、预后的关系。结果肝癌组织、癌旁组织、正常肝组织中PCDH8基因甲基化阳性率分别为61.25%、27.50%、0,RIZ1基因甲基化阳性率分别为70.00%、12.50%、2.00%,各组织间比较差异有统计学意义(P<0.05);有肝硬化、包膜侵犯、远处转移的患者PCDH8基因甲基化阳性率显著高于无肝硬化、包膜侵犯、远处转移的患者(P<0.05);有包膜侵犯的患者RIZ1基因甲基化阳性率显著高于无包膜侵犯患者(P<0.05);PCDH8、RIZ1基因甲基化阳性患者生存率低于PCDH8、RIZ1基因甲基化阴性患者(P<0.05)。COX单因素分析显示,临床分期、肝硬化、包膜侵犯、远处转移、PCDH8基因甲基化阳性、RIZ1基因甲基化阳性是肝癌患者总生存时间的影响因素(P<0.05);多因素分析显示,矫正其他因素后,PCDH8基因甲基化阳性是肝癌总生存时间的独立影响因素(P<0.05)。结论肝癌组织PCDH8、RIZ1基因甲基化状态与肿瘤发生、发展及预后相关,临床可考虑将基因甲基化状态辅助用于肝癌诊断、病情评估及预后预测中。Objective To explore the methylation status of PCDH8 and RIZ1 genes in liver cancer tissues and its clinical significance.Methods Eighty cases of liver cancer tissues,paracancerous tissues and 50 cases of normal liver tissues were selected as study specimens.The methylation status of PCDH8 and RIZ1 genes was detected by adopting the methylation-specific polymerase chain reaction(msPCR).And their relationship with clinicopathology and prognosis of liver cancer patients was analyzed.Results The positive rates of PCDH8 gene methylation in liver cancer tissues,paracancerous tissues and normal liver tissues were 61.25%,27.50%and 0 respectively,the positive rates of RIZ1 gene methylation were 70.00%,12.50%and 2.00%respectively,and the differences among groups were statistically significant(P<0.05).The positive rate of PCDH8 gene methylation in the patients with cirrhosis,capsule invasion and distant metastasis was significantly higher than that without cirrhosis,capsule invasion and distant metastasis(P<0.05).The methylation positive rate of RIZ1 gene in the patients with capsular invasion was significantly higher than that without capsular invasion(P<0.05).The survival rate in the patients with PCDH8 and RIZ1 gene methylation-positive was lower than that with methylation-negative(P<0.05).The COX univariate analysis showed that the clinical stage,cirrhosis,capsule invasion,distant metastasis,methylation-positive of PCDH8 and RIZ1 genes were the influencing factor of the total survival time in the patients with liver cancer(P<0.05).The multivariate analysis showed that after correcting other factors,the methylation positive of PCDH8 gene was an independent influencing factor of the total survival time of liver cancer(P<0.05).Conclusion The methylation status of PCDH8 and RIZ1 genes in liver cancer tissues is related with the occurrence,development and prognosis of the tumor.Clinically,the gene methylation status can be considered to be applied in liver cancer auxiliary diagnosis,disease severity assessment and prognosis

关 键 词：肝肿瘤 DNA甲基化 抑癌基因 预后 

分 类 号：R651[医药卫生—外科学]