金黄色葡萄球菌ATCC25923人工感染BALB/c雌鼠肾脏组织PRNP和PRND的表达变化研究  

Expression changes in PRNP and PRND in renal tissues of BALB/c female mice infected with Staphylococcus aureus ATCC25923

作　　者：孙康永杰 魏亚琴[2] 杨宇泽 万学瑞[1] 樊杰[1] 赵波 赵云海 郭容霞 王川[1] SUNKANG Yong-jie;WEI Ya-qin;YANG Yu-ze;WAN Xue-rui;FAN Jie;ZHAO Bo;ZHAO Yun-hai;GUO Rong-xia;WANG Chuan(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;Key Laboratory of Microbial Resources Exploitation and Application of Gansu Province,Institute of Biology,Gansu Academy of Sciences/Center of Anaerobic Microbes,Institute of Biology,Gansu Academy of Sciences,Lanzhou 730000,China;Beijing Animal Husbandry Station,Beijing 101011,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]甘肃省科学院生物研究所甘肃省微生物资源开发利用重点实验室/甘肃省科学院生物研究所厌氧微生物中心,兰州730000 [3]北京市畜牧总站,北京101011

出　　处：《中国人兽共患病学报》2020年第12期988-992,1013,共6页Chinese Journal of Zoonoses

基　　金：甘肃农业大学动物医学院学科建设基金(No.GSAU-XKJS-2018-074);甘肃农业大学2019年学生科研训练计划(No.SRTP,201903045);甘肃省科技计划项目重点研发计划(No.18YF1NA077);国家自然科学基金(No.31500067)。

摘　　要：目的为了研究细胞型朊蛋白和Doppel蛋白在金黄色葡萄球菌感染中的作用。方法本实验用金黄色葡萄球菌ATCC25923感染BALB/c雌鼠后,分别在1~7 d内解剖小鼠,进行组织抹片革兰氏染色和石蜡切片制作,最后采用荧光定量PCR检测PRNP和PRND在1~7 d中的相对表达量。结果金黄色葡萄球菌最先出现在肾脏,且对肾脏组织有损伤作用;PRNP和PRND基因在每个时段的表达量都高于空白对照组,其中PRNP在第1 d的表达量最高,为空白对照组的6.1倍,PRND在第2 d的表达量最高,为空白对照组的13.1倍。结论小鼠肾脏的PRNP和PRND的mRNA表达水平随着金黄色葡萄球菌感染的时间发生变化。To study the relationship between Staphylococcus aureus infection and the expression of cellular prion protein and Doppel protein,we infected female BALB/c mice with Staphylococcus aureus ATCC25923.The mice were euthanized,and different tissues were collected at baseline and 1-7 days post-infection.Tissue smear Gram staining and paraffin sectioning were performed,and the relative expression of the PRNP and PRND genes at baseline and 1-7 days post-infection was detected by fluorescence quantitative RT-PCR.Staphylococcus aureus first appeared in the kidneys,and damage to renal tissues was observed.The expression levels of PRNP and PRND in each period were higher than those at baseline.The expression of PRNP was highest on the first day(6.1 times that of the control),and the expression of PRND was highest on the second day(13.1 times that of the control).Our results suggest that the mRNA expression of PRNP and PRND changes with Staphylococcus aureus post-infection in the kidneys in mice.

关 键 词：金黄色葡萄球菌ATCC25923 PRNP基因 PRND基因 荧光定量PCR 

分 类 号：R378.1[医药卫生—病原生物学]