大鼠脑组织固有频率测定  

Measurement of natural frequency in rat brain

作　　者：卢新刚 尹露 安云 伍丹丹 杨诣坤 杨斌堂 严隽陶[1,2] LU Xingang;YIN Lu;AN Yun;WU Dandan;YANG Yikun;YANG Bintang;YAN Juntao(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;State Key Laboratory of Mechanical System and Vibration,Shanghai Jiaotong University,Shanghai 200240,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437 [2]上海交通大学机械系统与振动国家重点实验室,上海200240

出　　处：《中国医学物理学杂志》2020年第12期1560-1565,共6页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(30973806);国家自然科学基金青年科学基金(81603713);岳阳医院院级项目(KY11.30.57)。

摘　　要：目的:确定正常及常见中枢神经损伤、外周神经损伤后大鼠脑组织的固有频率,为经颅磁刺激、经颅声波刺激等非侵入性脑刺激技术的频率选择及相关动物实验的开展提供依据。方法:24只SD大鼠随机分为正常组、坐骨神经损伤(SNI)模型组和脑缺血再灌注(MCAO)模型组,每组8只大鼠。SNI模型组行坐骨神经钳夹伤术;MCAO模型组行脑缺血再灌注损伤术;两模型组造模7 d后,3组大鼠逐步小心地打开颅骨、硬脑膜,暴露大鼠脑部。频率源输出频率,激光传感实时频谱观察、时频分析方法,得出特性曲线,确定大鼠脑组织幅值B最大时出现的固有频率。结果:每组大鼠脑组织幅值最大时的固有频率峰值较为接近,正常组大鼠为(27.04±2.12)Hz,SNI模型组大鼠为(27.57±2.19)Hz,MCAO模型组大鼠为(26.44±2.27)Hz。SNI模型组与正常组大鼠固有频率差异无统计意义(P>0.05),MCAO模型组与正常组大鼠固有频率差异无统计意义(P>0.05),MCAO模型组与SNI模型组大鼠比较固有频率差异无统计意义(P>0.05)。结论:脑组织的固有频率是相对稳定的,在SNI周围损伤刺激时,以及MCAO这一中枢损伤刺激时,脑组织的固有频率不会发生较大差异。Objective To identify the natural frequencies of rat brain tissue before and after common central nerve injury and peripheral nerve injury with an aim of providing the basis for the frequency selection of non-invasive brain stimulation techniques such as transcranial magnetic stimulation and transcranial acoustic wave stimulation as well as for the development of relevant animal experiments.Methods 24 SD rats were randomly divided into normal group,sciatic nerve injury(SNI)model group and cerebral ischemia reperfusion(MCAO)model group,with 8 rats in each group.SNI model group underwent sciatic nerve clamp injury;MCAO model group underwent cerebral ischemia-reperfusion injury;7 days after the two model groups were modeled,the skulls and dura maters of three groups’rats were gradually opened,and the rats’brain units were carefully exposed.Frequency source output frequency,laser sensing real-time spectrum observation and time-frequency analysis method were used to obtain characteristic curve and identify the natural frequency that appears when the rat brain tissue amplitude B is maximum.Results The peaks of the natural frequency at the maximum amplitude of the brain tissue of each group of rats were relatively close.The normal group was(27.04±2.12)Hz,the SNI model group was(27.57±2.19)Hz,and the MCAO model group was(26.44±2.27)Hz.The natural frequency difference between the SNI model group and the normal group is not statistically significant(P>0.05),the difference between the MCAO model group and the normal group is not statistically significant(P>0.05),and the MCAO model group and the SNI model group also have no statistically significant difference.(P>0.05).Conclusion The natural frequency of brain tissue is relatively stable.There will be no big difference in the natural frequency of brain tissue even when stimulation of peripheral injury SNI and central damage MCAO was happened.

关 键 词：大鼠 脑 固有频率 神经损伤 

分 类 号：R318[医药卫生—生物医学工程] R-33[医药卫生—基础医学]