抗菌钛合金Ti6Al4V-6Cu表面构建成骨细胞膜片的实验研究  

Construction of osteoblast membrane sheet on thesurface of antibacterial titanium alloy Ti6Al4V-6Cu

作　　者：李抒缦 许雄程 何梦娇 钟泉[2,3] 陈超[2,3] 伍晓红[2,3] 骆凯[2,3] LI Shuman;XU Xiongcheng;HE Mengjiao;ZHONG Quan;CHEN Chao;WU Xiaohong;LUO Kai(Department of Stomatology,Geriatric Hospital of Fujian Province,Fuzhou 350003,China;Laboratory of Oral Tissue Engineering,Fujian Medical University,Fuzhou 350002,China;Department of Periodontology,Hospital of Stomatology Affiliated to Fujian Medical University,Fuzhou 350002,China)

机构地区：[1]福建省老年医院口腔科,福建福州350003 [2]福建医科大学口腔组织工程研究室,福建福州350002 [3]福建医科大学附属口腔医院牙周科,福建福州350002

出　　处：《中国医学物理学杂志》2020年第12期1589-1593,共5页Chinese Journal of Medical Physics

基　　金：福建省发改委第三批省级预算内投资计划项目;福建省自然科学基金(2017J01805);福建省教育厅面上项目(JA15199)。

摘　　要：目的:探讨大鼠颌骨成骨细胞在抗菌钛合金Ti6Al4V-6Cu表面构建细胞膜片的可行性。方法:体外培养大鼠颌骨成骨细胞,采用富含维生素C培养基在抗菌钛合金Ti6Al4V-6Cu表面构建细胞膜片(细胞膜片组),并以单纯培养基作对照(对照组),检测膜片形成过程中碱性磷酸酶(ALP)和成骨相关基因ALP、Ⅰ型胶原(Col-1)、骨形成蛋白2(BMP-2)的表达情况。结果:采用富含维生素C的培养基连续培养可在抗菌钛合金Ti6Al4V-6Cu表面成功构建成骨细胞膜片,该细胞膜片由多层细胞构成,富含胞外基质。相对于对照组,细胞膜片组的膜片形成过程中成骨细胞ALP活性及成骨相关基因ALP、Col-1、BMP-2的表达均显著增高。结论:在抗菌钛合金Ti6Al4V-6Cu表面可成功构建成骨细胞膜片,有望与Ti6Al4V-6Cu联合应用于引导性骨再生术。Objective To study the feasibility of constructing rat jaw osteoblast membrane sheet on the surface of antibacterial titanium alloy Ti6Al4V-6 Cu.Methods Osteoblasts were isolated and cultured in vitro from jaw bone of rats firstly.Osteoblast membrane sheets were then constructed on the surface of the antibacterial titanium alloy Ti6Al4V-6 Cu by using vitamin C-rich medium,and with those using pure medium as the control group,the expression of alkaline phosphatase(ALP)and osteoblastrelated genes ALP,typeⅠcollagen(Col-1),and bone morphogenetic protein(BMP-2)was detected and analyzed.Results Osteoblast membrane sheet can be successfully constructed on the surface of antibacterial titanium alloy Ti6Al4V-6 Cu by continuous culture using vitamin C-rich medium.The osteoblast membrane sheet was composed of multiple layers of osteoblasts and rich in extracellular matrix.Compared with the control group,the ALP activity of osteoblasts and the expression of osteoblast-related genes ALP,Col-1,BMP-2 were significantly increased in the osteoblast membrane sheet group during formation.Conclusions Osteoblast membrane sheets can be successfully constructed on the surface of antibacterial titanium alloy Ti6Al4V-6 Cu,so it is hopeful to combinedly apply Ti6Al4V-6 Cu to guided bone regeneration.

关 键 词：抗菌钛合金 Ti6Al4V-6Cu 金属 成骨细胞 细胞膜片 

分 类 号：R318[医药卫生—生物医学工程] R681[医药卫生—基础医学]