红梅汤制剂中槲皮素的鉴别与含量测定  

Identification and Content Determination of Quercetin in Hongmei Decoction

作　　者：张健民[1] 周婷婷 叶雅光 张俊杰 梁志云[1] 余香[1] ZHANG Jian-min;ZHOU Ting-ting;YE Ya-guang;ZHANG Jun-jie;LIANG Zhi-yun;YU Xiang(Jiangmen Xinhui Hospital of Traditional Chinese Medicine,Jiangmen,Guangdong 529100)

机构地区：[1]江门市新会区中医院,广东江门529100

出　　处：《按摩与康复医学》2020年第24期69-71,共3页Chinese Manipulation and Rehabilitation Medicine

基　　金：广东省江门市科技局项目,编号:2019H001。

摘　　要：目的:建立红梅汤制剂中槲皮素的定性定量检测方法。方法:槲皮素的鉴别采用薄层色谱法、含量测定采用高效液相色谱法(HPLC)。结果:红梅汤制剂中槲皮素的薄层色谱斑点清晰,含量测定槲皮素浓度在0.047~0.465μg/μL范围内线性关系良好,Y=48591X-4927(r=0.9998);平均加样回收率为97.76%,RSD=0.99%(n=6)。结论:此方法简便、精确,可作为该制剂的质量控制方法。Objective:To establish the quality control method of Hongmei decoction.Methods:TLC was used for qualitative analysis and HPLC for quantitative analysis.Results:The TLC spots were clear.The linear range of quercetin was 0.047~0.465μg/μL,and the regression equation was Y=48591 X-4927(r=0.9998)with an average recovery rate of 97.76%(RSD=0.99%,n=6).Conclusion:The method for identification and content determination of quercetin was simple and accurate,can be used for the quality control of Hongmei decoction.

关 键 词：红梅汤制剂 槲皮素 鉴别 含量测定 

分 类 号：R285.5[医药卫生—中药学]