HPLC同时测定川夏疏肝方中的三种成分  

Simultaneous Determination of Three Components in Chuanxia Shugan Formula by HPLC

作　　者：姚曼丽 YAO Man-li(Guangdong Second Traditional Chinese Medicine Hospital(Guangdong Research Institute of Traditional Chinese Medicine Manuracturing technology),Guangzhou,Guangdong 510095)

机构地区：[1]广东省第二中医院(广东省中医药工程技术研究院),广东广州510095

出　　处：《按摩与康复医学》2020年第24期76-78,81,共4页Chinese Manipulation and Rehabilitation Medicine

摘　　要：目的:建立川夏疏肝方中三种成分的HPLC同时测定的方法,准确评价该药物的质量。方法:使用HPLC法,色谱柱为Agilent SB C18(规格:250×4.6mm,粒径5μm),流动相为乙腈(A)-0.1%冰醋酸(B),梯度洗脱,程序为:0~15min,10%-10%A;15~30min,10%-25%A;30~45min,25%-75%A;45~50min,75%-10%A;检测波长254nm,流速1.0mL/min,柱温30℃,进样量10μL,检测指标为盐酸麻黄碱、杯苋甾酮、白术内酯I,共制备10批汤剂进行测定。结果:10批汤剂中,杯苋甾酮、盐酸麻黄碱、白术内酯I的含量分别为0.091~0.107mg/mL、0.232~0.259mg/mL、0.094~0.116mg/mL。结论:本研究建立了川夏疏肝方中三种成分的HPLC同时测定方法,该方法准确、快速、可重现,能够更为科学地评价该方的质量。Objective:To establish an HPLC simultaneous determination method for three components in Chuanxia Shugan formula,for the purpose of achieving accurate quality evaluation.Method:For HPLC analysis,the column used was Agilent SB C18(scale:250×4.6 mm,5μm particle size),mobile phase was acetonitrile(A)and 0.1%formic acid(B),with gradient elution,the program was:0~15 min,10%-10%A;15~30 min,10%-25%A;30~45 min,25%-75%A;45~50 min,75%-10%A;the detection wavelength was 254 nm,flow rate was 1.0 mL/min,column temperature was 30℃and the injection volume was 10μL,compounds namely ephedrina hydrochloridum,cyasterone and atractylenolide I,and 10 batches of decoction were analyzed.Results:In 10 batches of decoction,contents of ephedrina hydrochloridum,cyasterone and atractylenolide I were between 0.091~0.107 mg/mL,0.232~0.259 mg/mL,0.094~0.116 mg/mL,respectively.Conclusion:The study established an HPLC simultaneous determination method for three components in Chuanxia Shugan formula,which was accurate,fast and reproducible,and capable of providing more scientific quality evaluation for the formula.

关 键 词：川夏疏肝方 盐酸麻黄碱 杯苋甾酮 白术内酯I HPLC 同时测定 

分 类 号：R285.5[医药卫生—中药学]