柱后衍生高效液相色谱法测定鱼粉中组胺含量的研究  

Determination of histamine in fish meal by high-performance liquid chromatography with post-column derivatization

作　　者：郭红兵 朱磊 邱卫晓 邵伍军 GUO Hongbing;ZHU Lei;QIU Weixiao;SHAO Wujun(Zhejiang Hisun Pharmaceutical Co.Ltd,Taizhou Zhejiang 318000,China)

机构地区：[1]浙江海正药业股份有限公司,浙江台州318000

出　　处：《中兽医医药杂志》2020年第6期76-78,共3页Journal of Traditional Chinese Veterinary Medicine

摘　　要：建立柱后衍生高效液相色谱法测定鱼粉中组胺含量的方法。Waters Sunfire C18(4.6 mm×250 mm,5μm)色谱柱;流速1.0 mL/min;柱温30℃;流动相为甲醇-pH值2.6缓冲液(含有5 mmol/L辛烷磺酸钠和20 mmol/L磷酸氢二钾),梯度洗脱;采用荧光检测器(FLD),激发波长为338 nm,发射波长为445 nm。结果显示组胺在0.04~13.88μg/mL浓度下,相关系数r=1,线性良好;系统适用性、精密度、定量限的RSD均小于2%,加样回收率为93.62%~102.90%。说明该实验建立的方法操作简单,方法快速、可靠、专属性强,能够有效地检出鱼粉中的组胺。To determine the content of histamine in fish meal by high-performance liquid chromatography with Post-column derivatization.The determination was performed on a Waters Sunfire C18(4.6 mm×250 mm,5μm)column with mobile phase consisting of methanol-pH 2.6 buffer(containing 5 mmol·L^-1 Octane-1-sulfonic acid sodium and 20 mmol·L^-1 potassium dihydrogen phosphate)by gradient elution.The flow rate was 1.0 mL·min-1.The column temperature was 30℃.The samples were determined by HPLC with fluorescencedetector(excitation wavelength:338 nm,emission wavelength:445 nm) and quantified by external standard method.Good linearity between chromatographic peak area and histamine concentration ranged from 0.04 to 13.88μg·mL^-1 with the correlation coefficient of 1.The RSDs of system suitability,precision and limit of quantity were all less than 2%.The average recoveries were within the range of 93.62%~102.90%.The method was simple,rapid and reliable with high specificity,which could be used for the quality control of fish meal.

关 键 词：柱后衍生 HPLC 鱼粉 组胺 

分 类 号：S816.2[农业科学—饲料科学]