紫铆花素对PC12细胞氧化应激损伤的保护作用及对线粒体功能的影响研究  

Study on the Protective Effects of Butein on Oxidative Stress Injury of PC12 Cell and Its Effects on Mitochondrial Function

作　　者：刘珂娣 段佳林 苏晶 陶星茹 赵石 白杨[3] 卫培峰[3] 奚苗苗 LIU Kedi;DUAN Jialin;SU Jing;TAO Xingru;ZHAO Shi;BAI Yang;WEI Peifeng;XI Miaomiao(College of Pharmacy,Shaanxi University of TCM,Shaanxi Xianyang 712046,China;Dept.of Pharmacy,Xijing Hospital,Air Force Military Medical University,Xi’an 710032,China;National Drug Clinical Trial Institute,the Second Affiliated Hospital,Shaanxi University of TCM,Shaanxi Xianyang 712000,China;TANK Medicinal Biology Institute of Xi’an,Xi’an 710032,China)

机构地区：[1]陕西中医药大学药学院,陕西咸阳712046 [2]空军军医大学西京医院药剂科,西安710032 [3]陕西中医药大学第二附属医院国家药物临床试验机构,陕西咸阳712000 [4]西安天工生物医药研究所,西安710032

出　　处：《中国药房》2020年第24期2974-2981,共8页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81470174,No.81903832);陕西中医药大学第二附属医院学科创新团队建设项目(No.2020XKTD-A04)。

摘　　要：目的:研究紫铆花素对PC12细胞氧化应激损伤的保护作用及对线粒体功能的影响。方法:将大鼠PC12细胞分为正常对照组、模型组、溶剂对照组(1‰二甲基亚砜)和紫铆花素高、中、低浓度组(2、1、0.5μmol/L)。后4组给予相应试剂/药物干预24 h后,除正常对照组外的其余组均以100 m U/mL葡萄糖氧化酶诱导氧化应激模型。培养4 h后,检测细胞存活率、凋亡率和细胞内活性氧(ROS)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、三磷酸腺苷(ATP)、白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)水平或活性以及线粒体膜电位(MMP)变化。结果:与正常对照组比较,模型组细胞存活率和SOD、CAT、GSH-Px、ATP活性或水平均显著降低,凋亡率、ROS含量和MDA、IL-1β、TNF-α水平均显著增加(P<0.05或P<0.01),MMP明显降低。与模型组比较,溶剂对照组上述指标均无明显变化(P>0.05),紫铆花素高、中、低浓度组细胞存活率和SOD(除中、低浓度组外)、CAT、GSH-Px(除中、低浓度组外)、ATP(除低浓度组外)活性或水平均显著升高,凋亡率、ROS含量和MDA、IL-1β、TNF-α水平均显著降低(P<0.05或P<0.01),MMP明显增加。结论:紫铆花素可通过增强抗氧化酶活性,稳定线粒体功能,抑制氧化应激和炎症反应,增加能量生成,进而抑制神经细胞凋亡,最终发挥神经保护的作用。OBJECTIVE:To study the protective effects of butein on oxidative stress injury of PC12 cell and its effects on mitochondrial function.METHODS:Rats PC12 cells were divided into normal control group,model group,solvent control group(1‰dimethyl sulfoxide),butein high,medium and low concentration groups(2,1,0.5μmol/L).The latter 4 groups were given relevant reagent/medicine for intervention;24 h later,other groups were given 100 mU/mL glucose oxidase to induce oxidant stress model except for normal control group.After 4 h culture,cell survival rate,apoptosis rate,the levels or activities of ROS,MDA,SOD,CAT,GSH-Px,ATP,IL-1βand TNF-αas well as the change of MMP were detected.RESULTS:Compared with normal control group,cell survival rate,the levels or activities of SOD,CAT,GSH-Px and ATP were all decreased significantly,and apoptotic rate,the content of ROS,the levels of MDA,IL-1βand TNF-αwere all increased significantly(P<0.05 or P<0.01),while the MMP was decreased significantly.Compared with model group,above indexes of solvent control group had no significant change(P>0.05),cell survival rates,the levels or activities of SOD(except for medium and low concentration groups),CAT,GSH-Px(except for medium and low concentration groups),ATP(except for low concentration group)were increased significantly in butein high,medium and low concentration groups,while apoptotic rates,the content of ROS,the levels of MDA,IL-1βand TNF-αwere decreased significantly(P<0.05 or P<0.01),while the MMP were increased significantly.CONCLUSIONS:Butein can increase the antioxidant enzyme activity,stabilize mitochondrial function,inhibit oxidative stress and inflammationthus,increase energy generation inhibiting neuronal cell apoptosis ultimately exerting a neuroprotective effect.

关 键 词：紫铆花素 PC12细胞 氧化应激 线粒体功能 炎症 细胞凋亡 神经保护 

分 类 号：R285[医药卫生—中药学]