利用磷脂脂肪酸(PLFAs)生物标记法分析人工湿地根际土壤微生物多样性  

ANALYSIS ON MICROBIAL DIVERSITY IN THE RHIZOSPHERE OF CONSTRUCTED WETLANDS BY PHOSPHOLIPID FATTY ACIDS BIOMARKERS

作　　者：孙和泰[1] 华伟[1] 祁建民[1] 黄治军[1] 杜存浩 武晗琪 倪利晓[2] SUN He-tai;HUA Wei;QI Jian-min;HUANG Zhi-jun;DU Cun-hao;WU Han-qi;NI Li-xiao(Jiangsu Fangtian Electric Power Technology Co.,Ltd,Nanjing 211102,China;Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes,Ministry of Education,Hohai University,Nanjing 210098,China)

机构地区：[1]江苏方天电力技术有限公司,南京211102 [2]河海大学教育部浅水湖泊重点实验室,南京210098

出　　处：《环境工程》2020年第11期103-109,共7页Environmental Engineering

基　　金：国家自然科学基金(51779079);江苏省自然科学基金(BK20181313);江苏省水利科技项目(2018037);江苏方天电力项目(819049016)。

摘　　要：采用磷脂脂肪酸(PLFAs)生物标记法,分析了4组人工湿地分别栽种2种植物情况下植物根际土壤的微生物群落结构。结果表明:4组湿地植物根际土壤中PLFAs总量为1322~2769μg/g,PLFAs种类为18~30,湿地植物根际土壤中PLFAs主要包含饱和脂肪酸和单不饱和脂肪酸;植物根际土壤中细菌含量最高,其中好养菌含量显著高于厌氧菌。对PLFAs总量和湿地设计运行参数进行Pearson相关分析,PLFAs含量与基质孔隙率和进水水质呈显著正相关。The microbial community structure of rhizosphere soils in four groups of constructed wetlands were analyzed by phospholipid fatty acid(PLFAs)biomarker method.The results showed that the total amount of phospholipid fatty acid biomarker(PLFAs)in rhizosphere soil of four groups varied from 1322μg/g tO2769μg/g,and the range of PLFAs sorts number varied from 18 to 30.PLFAs in wetland plant rhizosphere soil mainly contained saturated fatty acids and monounsaturated fatty acids.The content of bacteria in plant rhizosphere soil was the highest,and the content of well-nourished bacteria was significantly higher than that of anaerobes.The total amount of PLFAs and the design and operation parameters of wetland were analyzed by Pearson correlation analysis.The results showed that the content of PLFAs was positively correlated with matrix porosity and influent water quality.

关 键 词：人工湿地 根际 微生物 磷脂脂肪酸 多样性 

分 类 号：X703[环境科学与工程—环境工程] S154.3[农业科学—土壤学]