一种确定斑马鱼胚胎接受的α粒子辐照剂量的方法  

Study on Dose Estimation Method of Alpha Irradiation on Zebrafish Embryos

作　　者：丁德馨 王慧敏[1,2] 赵维超 胡南 张燕斌 DING Dexin;WANG Huimin;ZHAO Weichao;HU Nan;ZHANG Yanbin(Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy,Hengyang,Hunan 421001,China;Hunan Province Key Laboratory of Green Development Technology for Extremely Low Grade Uranium Resources,University of South China,Hengyang,Hunan 421001,China)

机构地区：[1]铀矿冶生物技术国防重点学科实验室,湖南衡阳421001 [2]南华大学极贫铀资源绿色开发技术湖南省重点实验室,湖南衡阳421001

出　　处：《南华大学学报（自然科学版）》2020年第6期18-23,共6页Journal of University of South China：Science and Technology

基　　金：国防基础科研项目(JCKY2016403C001)。

摘　　要：为探究斑马鱼胚胎接受α粒子辐照剂量的计算方法,以模式生物斑马鱼为研究对象,使用能量为5.41 MeV的241Am为α粒子辐照源对受精后5 h脱绒毛膜的胚胎进行辐照处理,以12 μm厚的聚酯迈拉膜为固体核径迹探测器,利用正交实验筛选迈拉膜化学蚀刻的最优蚀刻条件,用显微镜观测迈拉膜上的粒子径迹数量,并通过计算胚胎相关参数,计算得到5hpf斑马鱼胚胎受到的最大辐照剂量值。结果分析表明,12 μm厚的聚酯迈拉膜化学蚀刻的最佳条件是蚀刻液KOH浓度6 mol/L,温度60 ℃,蚀刻时间3 h,此时粒子径迹轮廓清晰,大小适中。辐照5 min后,斑马鱼胚胎受到的最大剂量为约8.59 mGy,剂量率为约1.7 mGy/min。In order to investigate the dose of α particles irradiated on zebrafish embryos,the model organism zebrafish was used as the research object,and the 5 h post fertilization(5hpf) dechorionated embryos with depilatory membranes were placed in the bottom with a 2 mm hole diameter of a 3.5 cm petri dish,using a 12 μm thick polyester mylar film as the substrate and 241Am with an energy of 5.41 MeV as the irradiation source for 5 minutes,the chemical etching conditions were used for screening and the number of particle tracks on the polyester mylar film was observed.The estimation of the parameters yields the maximum radiation dose value received by the 5hpf zebrafish embryo.The results show that the best conditions for chemical etching of 12 μm thick polyester mylar film are 6 mol/L concentration of etching solution,60℃ of etching solution temperature and 3 h of etching time.At this time,the particle track outline is clear,the size is moderate,and it is easy to identify.After 5 minutes of irradiation,the maximum dose received by the zebrafish embryo is about 8.59 mGy,and the dose rate is about 1.7 mGy/min.

关 键 词：α粒子辐照 固体核径迹探测器 化学蚀刻 剂量计算 

分 类 号：TL815.7[核科学技术—核技术及应用]