长柄扁桃叶绿体基因组比较  

Comparison of complete chloroplast genome sequences of Amygdalus pedunculata Pall.

作　　者：段春燕[1] 张凯 段义忠[2] Chunyan Duan;Kay Zhang;Yizhong Duan(Agricultural College,Henan University of Science and Technology,Luoyang 471003,Henan,China;College of Life Sciences,Yulin University,Yulin 719000,Shaanxi,China)

机构地区：[1]河南科技大学农学院,河南洛阳471003 [2]榆林学院生命科学学院,陕西榆林719000

出　　处：《生物工程学报》2020年第12期2850-2859,共10页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(Nos.21675125,41601059);河南科技大学学科提升振兴A计划项目(No.13660001)资助。

摘　　要：蔷薇科桃属植物长柄扁桃Amygdalus pedunculata Pall.是我国重点发展的12种木本油料作物之一。将自然生长在陕西省榆林市毛乌素沙地的长柄扁桃进行高通量测序、组装得到了GenBank登录号为MG602257的长柄扁桃叶绿体基因组。与NCBI中其他两个长柄扁桃叶绿体基因组(MG869261和KY101153)进行序列比对发现:长柄扁桃叶绿体基因组均呈现典型的4分区域结构;基因组序列GC含量均是36.8%;总长度MG602257<MG869261<KY101153,分别为157851 bp、157873 bp和157948 bp;3个叶绿体基因组存在149个突变位点,包括93次替换事件、56次插入缺失事件、无小倒位事件。长柄扁桃叶绿体基因组密码子数目和RSCU值偏性分析具有极高的相似性。3个长柄扁桃叶绿体基因组在JLB/JSB/JSA/JLA四个边界有相同的基因类型和排列顺序,但在LSC/IRa边界JSA中,ndhF基因在MG602257和MG869261中的总长度相同,而在KY101153中总长度增加9 bp;ycf1基因横跨IRb/SSC边界区,基因长度在MG602257、MG869261和KY101153中分别为5657、5588、5684 bp,是造成全叶绿体基因长度差异的因素之一。基于全叶绿体基因组序列构建的系统发育进化树显示,3个长柄扁桃序列和一个榆叶梅序列聚在同一分支,说明榆叶梅和长柄扁桃亲缘关系较近。研究结果为长柄扁桃的叶绿体转基因工程、种质资源开发应用提供了基础实验数据。Amygdalus pedunculata Pall.is one of the 12 important woody oil crops in China.In this study,we determined the complete chloroplast genome sequence of A.pedunculata Pall.(MG602257)from Mu Us desert in Yulin city,Shaanxi province,China.The plastome was 157851 bp with 36.8%GC content.Comparisons among the plastomes of MG602257 and other two MG869261 and KY101153 from Genebank of NCBI showed that the total length of these chloroplast genomes was MG602257<MG869261<KY101153,the GC contents of these genomes were same,and 149 mutation events including 93 substitutions and 56 indels were accurately located.The codon numbers and RSCU values among the three were similar.They shared the same genes at the four boundaries of the LSC,SSC,IRa,and IRb regions.The lengths of ndhF at the LSC/IRa boundary in MG602257 and MG869261 were same while adjusting 9 bp in KY101153.The length of ycf1 gene at the SSC/IRb boundary in MG602257,MG869261 and KY101153 was 5657,5588 and 5684 bp,respectively,which was the one of the reasons for the size difference among the three plastomes.Phylogenetic analyses based on 13 complete plastomes of Rosaceae species confirmed close relationships between A.trilob and A.pedunculata.

关 键 词：长柄扁桃 蔷薇科 叶绿体基因组 密码子 高通量测序 

分 类 号：S565.9[农业科学—作物学]