槲皮素与DNA的相互作用  

Interaction between quercetin and DNA

作　　者：王弋 Yi Wang(Department of Marine Life,Ocean University of China,Qingdao 266003,Shandong,China)

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《生物工程学报》2020年第12期2877-2891,共15页Chinese Journal of Biotechnology

摘　　要：有机小分子与DNA相互作用机理研究已经成为药物作用机理研究与新药筛选的重要手段之一。槲皮素(Quercetin)是一种多羟基黄酮类化合物,具有抗癌、抗炎、抗菌、抗病毒、降糖降压、免疫调节及保护心血管的作用。实验研究的目的是发现与确认槲皮素与DNA之间是否具有相互作用,以及确定其相互作用的类型。根据荧光光谱法和共振散射荧光光谱法的分析结果,发现槲皮素与鲱鱼精DNA之间存在相互作用;使用紫外-可见分光光度法和荧光偏振分析,发现槲皮素与鲱鱼精DNA之间的相互作用模式不属于嵌插作用,而是沟槽嵌合或者静电相互作用;最后通过分子对接实验,成功佐证槲皮素与鲱鱼精DNA之间的相互作用模式是沟槽结合。该工作有利于理解槲皮素与DNA之间的体外作用方式,助力于相应疾病的药物开发。Studies on the interaction between small organic molecules and DNA are important means to explore drug mechanism and new drugs.Quercetin is a polyhydroxy flavone compound with activities such as anti-cancer,anti-inflammatory,antibacterial,antiviral,hypoglycemic and anti-hypertensive,immunomodulation and cardiovascular protection.Experimental studies aim at confirming if an interaction exists between quercetin and DNA,and determining the type of interaction.The interaction between quercetin and herring DNA can be detected by fluorescence spectrometry and resonance scattering fluorescence spectrometry analysis.The mode of the interaction between quercetin and herring DNA can be detected by UV-Vis spectrophotometry and fluorescence polarization analysis.This review helps understand the in vitro interaction between quercetin and DNA,and assist the development of drugs for corresponding diseases.

关 键 词：槲皮素 DNA 鲱鱼精DNA 相互作用 

分 类 号：R285[医药卫生—中药学]