副猪嗜血杆菌lpxL基因介导的生物被膜形成及黏附入侵能力的分析  

Analysis of Haemophilus parasuis lpxL gene mediated biofilm formation and adhesion invasion ability

作　　者：席振军 秦翠丽[1] 宋帅 张昆丽 勾红潮 李春玲 XI Zhen-jun;QIN Cui-li;SONG Shuai;ZHANG Kun-li;GOU Hong-chao;LI Chun-ling(Henan University of Science and Technology,Luoyang 471000,China;Institute of Animal Health,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)

机构地区：[1]河南科技大学,河南洛阳471000 [2]广东省农业科学院动物卫生研究所,广东广州510640

出　　处：《中国兽医科学》2020年第12期1542-1548,共7页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31772776);广东省现代农业产业技术体系创新团队项目(2019KJ119);广东省重点领域研发计划项目(2019B020217002);广东省自然科学基金项目(2020A1515010475)。

摘　　要：为了研究lpxL基因在副猪嗜血杆菌生物被膜形成及对细胞黏附入侵方面的作用,采用同源重组方法构建副猪嗜血杆菌lpxL基因缺失株H45-ΔlpxL;与野生株H45进行生物学特性比较。结果表明,lpx L基因缺失后,副猪嗜血杆菌的生长速度小于野生株;生物被膜合成量减小,对猪肾上皮细胞(PK-15)的黏附能力与入侵能力均有一定程度的减弱。结论,lpxL基因对副猪嗜血杆菌的增殖以及致病性都会产生一定作用。In order to study the role of lpx L gene in the formation of Haemophilus parasuis biofilm and cell adhesion and invasion,a homologous recombination method was used to construct Haemophilus parasuis lpx L gene-deficient strain H45-Δlpx L;and the wild strain H45 for biology comparison of characteristics showed that,after the deletion of the lpx L gene,the growth rate of Haemophilus parasuis was lower than that of the wild strain;the synthesis of biofilm was reduced,and the adhesion and invasion ability of pig kidney epithelial cells(PK-15)were weakened to a certain extent.The results showed that the lpx L gene had a certain effect on the growth performance and pathogenicity of Haemophilus parasuis.

关 键 词：副猪嗜血杆菌 lpxL 脂多糖 黏附入侵 

分 类 号：S852.612[农业科学—基础兽医学]