人类特异性基因TBC1D3抑制M1型巨噬细胞极化减轻瘢痕增生的实验研究  

Experimental Study on Human Specific Gene TBC1D3 Alleviate Hypertrophic Scar by Inhibiting M1 Macrophage Polarization

作　　者：徐爱娟[1] 郑江红[1] 杨松林[1] 邓辰亮[1] XU Aijuan;ZHENG Jianghong;YANG Songlin;DENG Chenliang(Department of Plastic Surgery,Shanghai Sixth People's Hospital,Shanghai 200233,China)

机构地区：[1]上海市第六人民医院整形外科,上海市200233

出　　处：《组织工程与重建外科杂志》2020年第6期467-470,共4页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国家自然科学基金项目(81370055)。

摘　　要：目的探索TBC1D3基因对增生性瘢痕形成的影响及作用机制。方法分别在TBC1D3转基因小鼠和对照组小鼠背部制备增生性瘢痕模型;应用RT-PCR技术分别在术前、术后2周、术后4周时,检测转基因小鼠与对照组小鼠TBC1D3基因表达水平;分别于术后2周、4周时收集各组瘢痕标本,采用Masson染色观察瘢痕组织病理学改变并评分;流式细胞仪检测术后2周、4周时转基因小鼠与对照组小鼠外周血巨噬细胞表型。结果术前、术后转基因小鼠中TBC1D3基因均明显表达,而对照组未见表达。术后2周、4周,转基因小鼠的瘢痕组织病理学评分均较对照组显著降低(P<0.05);术后2周、4周,转基因小鼠外周血中M1型巨噬细胞比例较对照组小鼠均显著降低(P <0.05)。结论 TBC1D3基因可以有效抑制增生性瘢痕形成,其机制可能是通过抑制M1型巨噬细胞极化实现的。Objective To explore the effect and mechanism of TBC1D3 gene on hypertrophic scar formation.Methods The hypertrophic scar models were made on the back of TBC1D3 transgenic mice and control mice respectively.Before operation,2 and 4 weeks after operation,the expression of TBC1D3 gene in scar tissue of each group was detected by RT-PCR.Scar samples were collected at 2 and 4 weeks after operation.The pathological changes of scar tissue were observed and scored by Masson staining.The phenotype of macrophages in peripheral blood of transgenic and control mice was detected by flow cytometry.Results TBC1D3 gene could be detected in transgenic mice before and after model preparation.No expression was found in the control group.At 2 and 4 weeks after operation,the pathological score of scar tissue in TBC1D3 transgenic mice was significantly lower than that in the control group(P<0.05),and the proportion of M1 macrophages in peripheral blood of TBC1D3 transgenicmice was significantly lower than that of wild-type mice(P<0.05).Conclusion TBC1D3 gene can effectively inhibit hypertrophic scar formation,which may be achieved by inhibiting M1 macrophage polarization.

关 键 词：增生性瘢痕 巨噬细胞 炎症 TBC1D3基因 

分 类 号：R619.6[医药卫生—外科学]