基于PET原理的FAM标记核酸适配体的汞离子检测研究  

Study on Mercury Ion Detection of FAM Labeled Aptamers Based on PET Principle

作　　者：张华伟 赵晗 于丽佳 ZHANG Hua-wei;ZHAO Han;YU Li-jia(National Center for Occupational Safety and Health,NHC,Beijing 102308,China)

机构地区：[1]国家卫生健康委职业安全卫生研究中心,北京102308

出　　处：《化学试剂》2020年第12期1460-1464,共5页Chemical Reagents

基　　金：国家自然科学基金资助项目(21907021);国家卫生健康委职业安全卫生研究中心自管课题项目(2020020、2020021)。

摘　　要：汞作为毒性最大的重金属之一,在环境中难以降解。利用光致诱导电子转移(PET)原理设计单端荧光标记核酸适配体用于汞离子检测研究。采用分子荧光光谱进行表征,激发波长490 nm,发射光谱范围为516~650 nm。在适配体5′末端修饰鸟嘌呤(G)碱基作为荧光淬灭基团,在3′末端修饰荧光素(FAM)基团。加入汞离子后,无规则卷曲单链形成茎环结构双链,G碱基与FAM接近产生PET效应荧光淬灭。在此体系中,线性范围为0~9 nmol/L,检测限为0.15 nmol/L,可在自来水中检测Hg^2+,回收率82.5%~98%。具有较高的灵敏度和特异性,操作方法简单。Mercury as one of heavy metals is high toxic to human health,which is difficult to degrade in the environment.Based on photoinduced electron transfer principle,a mercury detection probe consisting of a fluorescent labeled nucleic acid aptamer(HgAP)was designed.The fluorescence spectra was adopted with excitation wavelength 490 nm and emission wavelength range from 516 to 650 nm.Guanine(G)base was modified at the 5′-end of HgAP as fluorescence extraction group,and at the 3′-terminus as the FAM.After the addition of mercury ion,the irregular coil single chain formed the stem ring structure double chain,and the G base was close to FAM,resulting in PET fluorescence extraction.The linear range was 0~9 nmol/L and the detection limit was 0.15 nmol/L.This method could test Hg^2+in tap water with recovery rate range from 82.5%to 98%.It has high sensitivity and specificity,and the operation method is simple.

关 键 词：汞离子 适配体探针 光致诱导电子转移 茎环结构 荧光策略 

分 类 号：O657.31[理学—分析化学]