Cell-free synthetic biology in the new era of enzyme engineering  

作　　者：Nan Jiang Lianju Ma Yuan Lu 

机构地区：[1]College of Life Sciences,Shenyang Normal University,Shenyang 110034,China [2]Key Lab of Industrial Biocatalysis,Ministry of Education,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China

出　　处：《Chinese Journal of Chemical Engineering》2020年第11期2810-2816,共7页中国化学工程学报（英文版）

基　　金：supported by the National Key R&D Program of China(2018YFA0901700);the National Natural Science Foundation of China(Nos.21878173,21706144);the Natural Science Foundation of Beijing City(2192023)。

摘　　要：With the gradual rise of enzyme engineering,it has played an essential role in synthetic biology,medicine,and biomanufacturing.However,due to the limitation of the cell membrane,the complexity of cellular metabolism,the difficulty of controlling the reaction environment,and the toxicity of some metabolic products in traditional in vivo enzyme engineering,it is usually problematic to express functional enzymes and produce a high yield of synthesized compounds.Recently,cell-free synthetic biology methods for enzyme engineering have been proposed as alternative strategies.This cell-free method has no limitation of the cell membrane and no need to maintain cell viability,and each biosynthetic pathway is highly flexible.This property makes cell-free approaches suitable for the production of valuable products such as functional enzymes and chemicals that are difficult to synthesize.This article aims to discuss the latest advances in cell-free enzyme engineering,assess the trend of this developing topical filed,and analyze its prospects.

关 键 词：Cell-free synthetic biology Enzyme engineering Metabolic engineering 

分 类 号：Q814[生物学—生物工程]