四川部分地区半舍饲肉羊消化道蠕虫感染调查  

The Survey of Gastrointestinal Helminths Infection in Half Shed-feeding Sheeps and Goats in Some Areas of Sichuan Province

作　　者：叶勇刚[1,2] 肖璐 魏勇 康润敏[1,2] 于吉锋 张涛[3] 叶建强 曹冶[1,2] 谢晶 李兴玉[1,2] 潘梦[1,2] 林毅 孙文平[4] 戴卓建 廖党金[1,2] YE Yong-gang;XIAO Lu;WEI Yong;KANG Run-min;YU Ji-feng;ZHANG Tao;YE Jian-qiang;CAO Ye;XIE Jing;LI Xing-yu;PAN Meng;LIN Yi;SUN Wen-ping;DAI Zhuo-jian;LIAO Dang-jin(Sichuan Animal Science Academy,Chengdu 610066,China;Key Laboratory of Animal Genetic and Breeding of Sichuan Province,Chengdu 610066,China;Liangshan Institute of Animal Husbandry and Veterinary Sciences of Sichuan Province,Xichang 615042,China;Jiulong County Agriculture and Animal Husbandry Rural and Science and Technology Bureau of Sichuan Province,Jiulong 626200,China)

机构地区：[1]四川省畜牧科学研究院,四川成都610066 [2]动物遗传育种四川省重点实验室,四川成都610066 [3]四川省凉山州畜牧兽医科学研究所,四川西昌615042 [4]四川省九龙县农牧农村和科技局,四川九龙626200

出　　处：《中国兽医杂志》2020年第8期1-6,共6页Chinese Journal of Veterinary Medicine

基　　金：国家重点研发计划(2017YFD0501200);四川省财政运行专项(SASA2014CZYX009)。

摘　　要：为掌握四川半舍饲肉羊消化道蠕虫病的感染情况,试验采用廖氏法对四川6个市州的13个半舍饲羊场共计289份新鲜羊粪,进行了寄生虫种类和感染程度鉴定,随后对19只粪检阳性羊进行蠕虫剖检和成虫内转录间隔区(ITS)基因序列扩增测序。结果表明,四川半舍饲肉羊消化道蠕虫感染阳性率为64.36%(186/289),每个养殖场至少感染2种消化道蠕虫,其中线虫感染率最高,达60.55%(175/289),其次为片形吸虫、同盘吸虫和绦虫,阳性率分别为15.22%(44/289)、14.88%(43/289)和2.08%(6/289);线虫、片形吸虫和同盘吸虫的平均每克粪便虫卵数(EPG)分别为1197(56~28187)、527(51~1407)和413(63~1291);四川半舍饲肉羊流行12种消化道蠕虫,即肝片形吸虫、大片形吸虫、鹿同盘吸虫、鹿列叶吸虫、捻转血矛线虫、羊夏伯特线虫、粗纹食道口线虫、羊毛首线虫、达氏背板线虫、普通奥斯特线虫、贝氏莫尼茨绦虫和扩展莫尼茨绦虫。生产上需加大对此类寄生虫病尤其线虫病的防控。This study aimed at evaluating the status of gastrointestinal helminths infection in half shed-feeding fleshy sheep in Sichuan.A totle of 289 fresh feces excreted by fleshy sheeps feeded in 13 half shed-feeding farms were examined by egg counting method created by Liao Dang-jin.Then 19 positive sheeps or goats infected by gastrointestinal helminths were slaughtered for parasitological necropsy and the ITS gene of adult helminths were amplified and sequenced.The result showed that the infection rate of gastrointestinal helminths in half shed-feeding fleshy sheep was 64.36%(186/289)and at least two kinds of gastrointestinal helminths were found in each farm,among which the infection rate of nematodes was the highest 60.55%(175/289).Followed by Fasciola spp.,Paramphistomum spp.and Tapeworms,the rates were 15.22%(44/289),14.88%(43/289)and 2.08%(6/289)respectively.The eggs counting revealed the average EPG of nematodes,Fasciola spp.and Paramphistomum spp.were 1197(56~28187),527(51~1407)and 413(63~1291),respectively.Through morphological and molecular identification to adult heminths,12 species of digestive tract helminths parasited in half shed-feeding fleshy sheep were identified in Sichuan which were Fasciola hepatica,Fasciola gigantica,Paramphistomum cervi,Ogmocotyle sikae,Haemonchus contortus,Chabertia Ovina,Oesophagostomum asperum,Trichuris ovis,Tedadorsagia davtiani,Ostertagia circumcincta,Moniezia benedeli and Moniezia expansa.It is necessary for farms to prevention and control such parasitic diseases,especially nematodes.

关 键 词：半舍饲 肉羊 消化道蠕虫 

分 类 号：S855.9[农业科学—临床兽医学]