五倍子与抗菌药联合用药对产ESBLs大肠埃希菌的作用研究  

Antibiotic Effect of Galla chinensis Extract Combined with Antibacterial Drugs against ESBLs-producing E.coli

作　　者：王婧[1] 雷春娟[2] 濮鑫怡 徐泽 陈海峰[1] WANG Jing;LEI Chun-juan;PU Xin-yi;XU Ze;CHEN Hai-feng(Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China;China Animal Disease Control Center,Beijing 1000126,China)

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300 [2]中国动物疫病预防控制中心,北京朝阳100126

出　　处：《中国兽医杂志》2020年第8期48-52,共5页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目(31902317);江苏省高校自然科学基金项目(18KJB230003);江苏农牧科技职业学院校级课题(NSF201909);大学生创新创业训练计划(201912806028Y);江苏高校“青蓝工程”资助项目。

摘　　要：为研究五倍子与抗菌药联合使用对产超广谱β-内酰胺酶(ESBLs)大肠埃希菌的作用,本试验从临床分离的菌株中筛选产ESBLs大肠埃希菌并进行基因型鉴定,采用微量棋盘法测定了五倍子提取物与抗菌药联合使用的联合抑菌浓度指数(FICI)。结果表明,本试验共筛选到产ESBLs大肠埃希菌26株,阳性率为32.1%,鉴定到2株SHV、OXA和CTX-M-25均阳性的产ESBLs大肠埃希菌。五倍子提取物对这2株细菌的最低抑菌浓度(MIC)分别为32μg/mL和64μg/mL;棋盘试验结果表明,五倍子与硫酸庆大霉素和环丙沙星联用时表现为协同作用,与头孢噻呋钠联用为协同作用或无关作用,而与阿莫西林、盐酸左氧氟沙星和氟苯尼考联用表现为无关作用。In order to investigate the antibacterial effect of the extract from Galla chinensis combined with antibiotics on ESBLs-producing E.coli,ESBLs-producing E.coli was screened from clinically isolated strains and genotypes were identified in this study.Then the FIC index of Galla chinensis extract combined with antibiotic was determined by micro chessboard method.The results showed that the 26 strains of ESBLs-producing E.coli were screened in this study,with a positive rate of 32.1%.And there were two strains SHV,OXA and CTX-M-25 positive ESBLs-producing E.coli,and the MICs of gallnut extract for the two strains of bacteria were 32 and 64μg/mL,respectively.The gallnut extract combined with gentamycin sulfate and ciprofloxacin showed a synergistic effect.Combined with ceftiofur sodium,it showed a synergistic or an additive effect.Combined with amoxicillin,levofloxacin hydrochloride and florfenicol showed an indifferent effect.

关 键 词：超广谱Β-内酰胺酶 大肠埃希菌 五倍子 抗菌药 联合用药 

分 类 号：S859[农业科学—临床兽医学]