食源性金黄色葡萄球菌相关毒力基因的时序性表达规律研究  

The Temporal Expression of Staphylococcus aureus in Foodborne Related Virulence Genes

作　　者：魏歆 林芳明 雷生妍 张红见[1] 贾彩惠 葛丽萍 赵静[1] WEI Xin;LIN Fang-ming;LEI Sheng-yan;ZHANG Hong-jian;JIA Cai-hui;GE Li-ping;ZHAO Jing(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Qinghai Provincial Food Inspection and Testing Institute,Xining 810008,China;The Central Station of Animal Husbandry Technology Promotion,Xi’an 710016,China)

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]青海省食品检验检测院,青海西宁810008 [3]陕西省畜牧技术推广总站,陕西西安710016

出　　处：《中国兽医杂志》2020年第8期73-76,I0008,共5页Chinese Journal of Veterinary Medicine

基　　金：青海省农牧厅项目(NMSY-2016-08);国家自然科学基金(31560695,31560701);青海省科技厅项目(2020-HZ-801)。

摘　　要：为了研究西宁市不同区域食品中主要金黄色葡萄球菌肠毒素(SE)与耐热核酸酶(nuc)基因的时序性表达规律及二者之间的相关性,本试验利用实时荧光定量多聚酶链式反应(RT-qPCR)技术在mRNA水平对金黄色葡萄球菌A、B、E三种SE基因及nuc基因在不同生长阶段的相对表达量进行研究。结果表明:不同基因型的金黄色葡萄球菌在不同来源、不同种类的食品中的表达水平有显著差异。SEA与nuc基因的表达规律一致;SEB和SEE与nuc基因的表达规律则不一致,且SEB高表达。本试验结果为金黄色葡萄球菌毒力机制研究与食品质量安全控制提供了参考。In order to study the temporal expression of the enterotoxin(SEA、SEB and SEE)from Staphylococcus aureus and heat-resistant nuclease(nuc)genes from foodborne in Xining areas,their temporal expression at different growth stages of mRNA and the correlation between SE and nuc genes by RT-qPCR were studied.The results showed that it was significantly different in food sources and species from in genotypes of S.aureus.It was consistent when comparing SEA with nuc gene expression,while it was different when comparing SEB and SEE with nuc gene expression of S.aureus,SEB was highly expressed.It would be valuable to further study the pathogenic mechanism and the control of food safety of S.aureus in foodborne.

关 键 词：金黄色葡萄球菌 肠毒素 耐热核酸酶 RT-QPCR 

分 类 号：R378.1[医药卫生—病原生物学]