取食不同寄主植物对草地贪夜蛾幼虫体内3类酶活性的影响  

Effects of host plants on activities of three groups of enzymes in Spodoptera frugiperda(Smith)larvae

作　　者：鲁智慧 和淑琪 郭晋 陈亚平 胡雪琼[3] 李浩 谢琦 桂富荣 LU Zhi-hui;HE Shu-qi;GUO Jin;CHEN Ya-ping;HU Xue-qiong;LI Hao;XIE Qi;GUI Fu-rong(Plant Protection College,Yunnan Agricultural University/State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan,Kunming 650201,China;Yunnan Agricultural Environmental Protection Monitoring Station,Kunming 650201,China;Yunnan Climate Center,Kunming 650034,China;Yunnan Plateau Characteristic Agriculture Industry Research Institute,Kunming 650201,China)

机构地区：[1]云南农业大学植物保护学院/省部共建云南生物资源保护与利用国家重点实验室,昆明650201 [2]云南省农业环境保护监测站,昆明650201 [3]云南省气候中心,昆明650034 [4]云南省高原特色农业产业研究院,昆明650201

出　　处：《南方农业学报》2020年第10期2461-2469,共9页Journal of Southern Agriculture

基　　金：国家重点研发计划项目(2019YFD0300101);云南省科技重大专项(2019ZG00910)。

摘　　要：【目的】明确取食不同寄主植物对草地贪夜蛾(Spodoptera frugiperda)幼虫体内消化酶、保护酶和解毒酶活性的影响,为进一步探究草地贪夜蛾的寄主选择及适应机制提供理论依据。【方法】在室内用玉米、小麦、马铃薯和烟草4种寄主植物叶片饲喂草地贪夜蛾初孵幼虫至5龄,测定取食不同寄主植物后草地贪夜蛾体内消化酶(脂肪酶、胃蛋白酶和α-淀粉酶)、保护酶[超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)]和解毒酶[谷胱甘肽S-转移酶(GST)、羧酸酯酶(CarE)、多功能氧化酶(MFO)和细胞色素P450(CYP450)]的活性变化,并比较4种寄主植物叶片次生代谢物(总酚、单宁和类黄酮)及营养物质(可溶性糖和可溶性蛋白质)含量,分析草地贪夜蛾幼虫体内酶活变化与寄主植物次生代谢物及营养物质含量的相关性。【结果】取食马铃薯叶片的草地贪夜蛾幼虫体内脂肪酶和胃蛋白酶活性均高于或显著高于取食其余3种寄主植物叶片的草地贪夜蛾幼虫(P<0.05,下同),而取食烟草叶片的草地贪夜蛾幼虫体内α-淀粉酶活性(0.48±0.08 U/mg)最高,显著高于取食其他3种寄主植物叶片的草地贪夜蛾幼虫。取食马铃薯叶片的草地贪夜蛾幼虫体内SOD和POD活性均显著高于取食其他3种寄主植物叶片的草地贪夜蛾幼虫,而取食烟草叶片的草地贪夜蛾幼虫体内CAT活性最高(225.54±51.56 U/mg),是取食玉米叶片草地贪夜蛾幼虫的2.71倍。取食马铃薯和烟草叶片的草地贪夜蛾幼虫体内GST、CYP450和MFO活性均高于取食玉米和小麦叶片的草地贪夜蛾幼虫,其中取食马铃薯和烟草叶片的草地贪夜蛾幼虫体内GST活性(100.50±4.71和98.69±6.21 U/mg)显著高于取食玉米和小麦叶片的草地贪夜蛾幼虫GST活性(54.85±1.94和70.62±4.04 U/mg);而CarE活性在取食烟草叶片的草地贪夜蛾幼虫体内最高,为184.79±9.05 U/g。4种寄主植物叶片中总酚、单宁�【Objective】To clarify the effects of different host plants on activities of threegroups of enzymes(digestive enzymes,protective enzymes and detoxification enzymes)in fall armyworm(FAW),Spodoptera frugiferda(Smith)larvae,and provide theoretical basis for further study on host selection and adaptation mechanism of S.frugiferda.【Method】Leaves of four host plants,including maize,wheat,potato and tobacco,were used to feed FAW larvae from the 1st instar to the 5th instar,and the activities of digestive enzymes(lipase,pepsin,andα-amylase),protective enzymes[superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)]and detoxification enzymes[glutathione S-transferase(GST),carboxylesterase(CarE),multifunctional oxidase(MFO),cytochrome P450(CYP450)]in the 5th instar of S.frugiperda were determined.The secondary metabolites(total phenols,tannins,flavonoids)and nutrients substance(soluble sugars,soluble proteins)content in the four host plant leaves were tested,and the correlation between enzyme activities in FAW larvae and the contents of secondary metabolites and nutrients in host plants was analyzed.【Result】The activities of lipase and pepsin inlarvae fed on potato leaves were higher or significantly higher than those in larvae fed on the other three host plant leaves(P<0.05,the same below),while theα-amylase activity in larvae fed on tobacco leaves(0.48±0.08 U/mg)was highest,which was significantly higher thanlarvae fed onother three host plants.The activities of SOD and POD in larvae fed on potato leaves were significantly higher than those in larvae fed on the other three host plant leaves.The CAT activity(225.54±51.56 U/mg)in larvae fed on tobacco leaves was the highest,which was as 2.71 times as that in larvae fed on maize;the activities of GST,CYP450 and MFO in larvae fed on potato and tobacco leaves were higher than those in larvae fed on maize and wheat leaves,the GST activity in larvae fed on potato and tobacco leaves(100.50±4.71 U/mg and 98.69±6.21 U/mg)were significantly higher than in larva

关 键 词：草地贪夜蛾 消化酶 保护酶 解毒酶 寄生植物 次生代谢物 营养物质 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]