姜黄与紫色姜提取物光照处理后的抗菌活性研究  

Studies on Antimicrobial Activity of Curcuma Longa and Zingiber Purpureum Extracts after Illumination

作　　者：黎晓菊 庹呈杰 周明曦 张科涛 张冰 黄之镨 张晓梅 赵庆 LI Xiaoju;TUO Chengjie;ZHOU Mingxi;ZHANG Ketao;ZHANG Bing;HUANG Zhipu;ZHANG Xiaomei;ZHAO Qing(unnan University of Chinese Medicine,Kunming 650500,China;hengdu University of TCM,Chengdu 610072,China)

机构地区：[1]云南中医药大学,云南昆明650500 [2]成都中医药大学,四川成都610072

出　　处：《云南中医学院学报》2020年第5期6-10,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(21662048、81460533、81060262);云南省科技厅-云南中医学院应用基础研究联合专项[2017FF116(-011)]。

摘　　要：目的研究姜黄和紫色姜光照前后抗菌活性的变化。方法采用纸片扩散法,测试姜黄与紫色姜提取物在光照(LED灯照射2 h或4 h)前后,对20种病原菌抗菌活性的变化。采用薄层色谱法检测姜黄和紫色姜提取液光照后生成的过氧化物与还原性物质,以判断是否发生了光化学反应。结果光照后姜黄与紫色姜提取物的抗菌活性都明显增强。姜黄与紫色姜提取液光照后均产生新的成分,这表明发生了光化学反应。结论在姜黄素类成分的催化下,姜黄与紫色姜的化学成分在光照时发生光化学反应,生成了抗菌活性更高的产物。Objective The changes of antimicrobial activities of Curcuma longa and Zingiber Purpureum before and after illumination were studied.Methods Disk diffusion test was applied to detect the antimicrobial activity of Curcuma longa and Zingiber Purpureum extracts against 20 kinds of microbial pathogens before and after illumination.TLC was applied to detect peroxide products and reductive products in the extracts of Curcuma longa and Zingiber Purpureum after illumination,by which whether the photochemical reaction occurred were determined.Results The antimicrobial activities of the two extracts increased significantly after illumination.New products were detected in the two extracts after illumination,which indicated that the photochemical reaction had occurred.Conclusion Under the catalysis of curcuminoids,some components in Curcuma longa and Zingiber Purpureum underwent photochemical reaction under illumination.As a result,components with higher antimicrobial activity were generated.

关 键 词：姜黄 紫色姜 姜黄素类 抗菌活性 光化学反应 

分 类 号：R285.5[医药卫生—中药学]