UPLC-MS-MS法同时测定玉屏风颗粒中6种皂苷类成分  

Simultaneous determination of 6 saponins in Yupingfeng granules by UPLC-MS-MS

作　　者：胥爱丽 钟惠娴 杨小龙 毕晓黎 肖观林 陈昭 江洁怡 XU Aili;ZHONG Huixian;YANG Xiaolong;BI Xiaoli;XIAO Guanlin;CHEN Zhao;JIANG Jieyi(Guangdong Province Engineering Technology Research Institute of T.C.M,Guangzhou 510095,China;Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine,Guangzhou 510095,China;Guangdong Yifang Pharmaceutical Co. Ltd.,Foshan 528244,China;Guangzhou The fifth Clinical Medical College,Guangzhou University of traditional Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广东省中医药工程技术研究院,广东广州510095 [2]广东省中医药研究开发重点实验室,广东广州510095 [3]广东一方制药有限公司,广东佛山528244 [4]广州中医药大学第五临床医学院,广东广州510405

出　　处：《广东药科大学学报》2020年第6期791-796,共6页Journal of Guangdong Pharmaceutical University

基　　金：广东省名优中成药二次开发项目“名优中成药玉屏风颗粒的二次开发”(粤中医办函[2017]19号);广东省科技计划项目“创新中药研发平台建设”(2017A070701017)。

摘　　要：目的建立同时测定玉屏风颗粒中6种皂苷类成分的快速方法,以更全面地评价玉屏风颗粒的质量。方法采用UPLC-MS-MS法,ESI离子源,色谱柱为Waters ACQUITY UPLC HSS T3 C18(1.8μm,2.1 mm×100 mm),流动相为乙腈-0.1%甲酸(梯度洗脱),流速为0.30 mL/min,柱温为30℃,进样量为2μL。结果黄芪甲苷、黄芪皂苷Ⅲ、黄芪皂苷Ⅱ、异黄芪皂苷Ⅱ、黄芪皂苷Ⅰ和异黄芪皂苷Ⅰ质量浓度分别在0.1775~3.55、0.0625~1.25、0.298~5.96、0.1575~3.15、0.2025~4.05、0.29~5.8μg/mL范围内与峰面积呈良好的线性关系,平均回收率分别为100.64%、97.66%、100.89%、104.57%、101.73%、98.19%,RSD分别为3.54%、1.93%、2.63%、2.42%、3.35%、2.57%。结论本文方法同时对玉屏风颗粒的6个皂苷类成分进行分析,方法简便、快速、准确,为有效评价玉屏风颗粒质量提供实验依据。Objective To establish a simultaneous determination method for 6 saponins in Yupingfeng granules,for the purpose of more precisely evaluation of its quality information.Methods UHPLC-MS-MS was adopted on a Waters ACQUITY UPLC HSS T3 C18(1.8μm,2.1 mm×100 mm)column,and acetonitrile-0.1%formic acid was used as the mobile phase by gradient elution.The flow rate was 0.3 mL/min,the column temperature was set at 30℃,and the injection volume was 2μL.Results Astragaloside,AstragalosideⅢ,AstragalosideⅡ,IsostragalosideⅡ,AstragalosideⅠand IsostragalosideⅠhad a good linearity relationship between 0.1775-3.55,0.0625-1.25,0.298-5.96,1575-3.15,0.2025-4.05,0.29-5.8μg/mL,and the average recoveries were 100.64%(RSD=3.54%),97.66%(RSD=1.93%),100.89%(RSD=2.63%),104.57%(RSD=2.42%),101.73(RSD=3.35%)and 98.19%(RSD=2.57%),respectively.Conclusion The method of simultaneous determination for 6 saponins in Yupingfeng granules is simple,rapid and accurate,which provides experimental basis for the quality evaluation of Yupingfeng granules.

关 键 词：玉屏风颗粒 皂苷 UPLC-MS-MS 

分 类 号：R927.2[医药卫生—药学]