HPLC法测定清咽丸中靛蓝的含量  

Determination of Indigo in Qingyan Pills by HPLC

作　　者：冯娟[1] 郭均怡 覃亮 FENG Juan;GUO Junyi;QIN Liang(GuangDong Food and Drug Vocational College,Guangdong Guangzhou,510520;College of food and pharmaceutical engineering,Zhaoqing University,Guangdong Zhaoqing 526061)

机构地区：[1]广东食品药品职业学院,广东广州510520 [2]肇庆学院食品与制药工程学院,广东肇庆526061

出　　处：《生物化工》2020年第6期89-91,共3页Biological Chemical Engineering

基　　金：2017年广东省本科高校教学质量与教学改革项目-重点专业建设项目(NO.359)。

摘　　要：目的:采用高效液相色谱法测定清咽丸中靛蓝成分的含量。方法:选取Agilent 5 TC-C18(250 mm×4.6 mm,5μL)色谱柱,乙腈∶水=42∶58为流动相,流速为1.0 mL/min,柱温为30℃,检测波长为286 nm,测定清咽丸中靛蓝的含量。结果:该方法专属性强,精密度高,稳定性、重复性好,加样回收率高。结论:HPLC法测定清咽丸中的靛蓝方法准确、可控,能简便有效地监测清咽丸的质量。Objective To establish a HPLC method for the determination of indigo in Qingyan pills.Method:Agilent 5 TC-C18(250 mm×4.6 mm,5μL)chromatographic column with acetonitrile∶water=42∶58 as mobile phase was used to determine the content of indigo in Qingyan pills.The flow rate was 1.0 mL/min,column temperature was 30℃,detection wavelength was 286 nm.Results:The method has strong specificity,high precision,good stability and repeatability,and high sample recovery rate.Conclusion:The HPLC method for the determination of indigo in Qingyan Pills is accurate and controllable,and can easily and effectively control the quality of Qingyan Pills.

关 键 词：高效液相色谱法 清咽丸 靛蓝 含量测定 

分 类 号：R96[医药卫生—药理学]