参附注射液对内毒素休克大鼠肺组织表达PPARγ和TNFα的干预作用  

Effect of Shenfu Injection on the Expression of PPARγand TNFαin Lung Tissue of Rats with Endotoxic Shock

作　　者：褚春薇 艾飞 陈向云 郭俊峰 杨毅 刘霞 CHU Chunwei;AI Fei;CHEN Xiangyun;GUO Junfeng;YANG Yi;LIU Xia(School of Basic Medicine,Guizhou University of Chinese Medicine,Guiyang 550025,China;Second Clinical Medical College,Guizhou University of Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学基础医学院,贵阳550025 [2]贵州中医药大学第二临床医学院,贵阳550025

出　　处：《实验动物科学》2020年第5期14-18,共5页Laboratory Animal Science

基　　金：国家自然科学基金地区基金项目(No.81760738)。

摘　　要：目的观察内毒素休克大鼠肺组织中过氧化物酶体增殖物激活受体γ(PPARγ)和肿瘤坏死因子α(TNFα)的表达情况及参附注射液的干预作用。方法经腹腔注射脂多糖(LPS)建立内毒素休克SD大鼠模型,用低、中、高剂量参附注射液进行治疗,观察肺组织病理改变,检测肺组织中PPARγ和TNFα的表达量,同时检测血浆中TNFα和IL-1β的水平。结果模型组大鼠具有典型的急性肺损伤表现;肺组织中PPARγ的转录和表达(P<0.01)量均显著下调,TNFα的表达明显增加(P<0.01);血浆中TNFα和IL-1β水平明显上升(P<0.01)。与模型组比较,参附注射液改善肺组织充血、水肿及炎性细胞浸润;呈剂量依赖性上调肺组织中PPARγ的转录和表达,下调TNFα的表达;同时抑制血浆中炎症介质TNFα和IL-1β水平(P<0.01)。结论参附注射液保护内毒素休克大鼠肺组织,其作用机制可能与上调PPARγ从而抑制炎症介质的产生有关。Objective To investigate expression of peroxisome proliferator activated receptor gamma(PPARγ)and tumor necrosis factor alpha(TNFα)in lung tissue of rats with endotoxic shock and the intervention effect of Shenfu injection.Method SD rats were selected to establish the animal model of endotoxin shock by intraperitoneal injection of lipopolysaccharide(LPS),followed by the treatment with shenfu injection(low,medium and high doses).The pathological changes of lung tissue were observed.The transcription and expression of PPARγand TNFαin lung tissue and secretion of TNFαand IL-1βin plasma were detected.Result There were typical acute lung injury in the rats of LPS group,the transcription and expression of PPARγin lung tissue were significantly decreased(P<0.01),the expression of TNFαwas obviously increased(P<0.01),and the levels of TNFαand IL-1βin plasma were significantly increased(P<0.01).However,compared with LPS group,Shenfu Injection relieved pulmonary congestion,edema and inflammatory cell infiltration,increased the transcription and expression of PPARγin lung tissue in a dose-dependent manner,decreased TNFαexpression,and inhibited the levels of inflammatory factors TNFαand IL-1βin plasma as well(P<0.01).Conclusion Shenfu injection can protect lung tissue of rats with endotoxic shock,and its mechanism may be related to up regulating PPARγand inhibiting the production of inflammatory mediators.

关 键 词：参附注射液 过氧化物酶体增殖物激活受体 急性肺损伤 内毒素休克 炎症介质 

分 类 号：R285.5[医药卫生—中药学]