制作简易实验动物雾化箱建立小鼠哮喘模型研究  

Study on Building Mouse Asthma Model with Self-designed Experimental Animal Atomization Box

作　　者：田丹[1] 许虎峰[1] TIAN Dan;XU Hufeng(Experimental and Transcriptional Research Center,Beijing Friendship Hospital,Capital Medical University,Beijing Clinical Medical Institute,Beijing Key Laboratory of Tolerance Induction and Organ Protection inTransplantation,Immunology Research Center for Oral and Systemic Health,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院科研实验中心,北京市临床医学研究所,移植耐受与器官保护北京市重点实验室,王松灵院士口腔与全身健康免疫研究实验室,北京100050

出　　处：《实验动物科学》2020年第5期71-73,共3页Laboratory Animal Science

基　　金：北京友谊医院“友谊种子计划”(No.YYZZ2017A23)。

摘　　要：目的应用简单易操作的方法将家用型医疗雾化吸入装置改造为实验动物雾化箱,为实验动物呼吸道疾病的研究提供便利。方法利用家用型雾化器和动物实验室常见的独立通气鼠笼组合改造为实验动物雾化箱。应用自制实验动物雾化箱和鸡卵清白蛋白建立小鼠过敏性哮喘模型。结果实验动物雾化箱能同时为1~20只小鼠实验快速稳定地产出雾化吸入气体,利用该雾化箱成功建立起小鼠过敏性哮喘模型。结论我们利用该实验动物雾化箱建立小鼠过敏性哮喘模型并观察到明显的肺组织炎症表现。该方法为简便和低成本地进行实验动物雾化实验提供具有操作性的指导。Objective To design an experimental animal atomization box with a household medical atomizer and facilitate airway disease research in experimental animals.Method A household atomizer and a separate ventilating squirrel cage were combined and used to transform into an experimental animal atomization box.We built a mouse asthma model with the self-designed atomization box and ovalbumin.Result The atomization box could produce enough fog for 1-20 mice experiment rapidly.We successfully built the mouse asthma model with the atomization box.Conclusion We applied the atomization box to build the mouse allergic asthma model and observed significant lung tissue inflammation.This method provides operational guide for experimental animal inhalation experiment with convenience and low cost.

关 键 词：雾化箱 动物模型 哮喘 小鼠 

分 类 号：Q95-337[生物学—动物学]