高通量筛选技术在体外线粒体毒性评价中的应用  

Application of high throughput screening techniques in evaluation of mitochondrial toxicity in vitro

作　　者：虞慧华 蔺红伟 陆文铨 陈万生 朴淑娟 YU Huihua;LIN Hongwei;LU Wenquan;CHEN Wansheng;PIAO Shujuan(Department of Pharmacy,Changhai Hospital,Naval Medical University,Shanghai 200433,China;Department of Pharmacy,Changzheng Hospital,Naval Medical University,Shanghai 200003,China)

机构地区：[1]海军军医大学长海医院药学部,上海200433 [2]海军军医大学长征医院药学部,上海200003

出　　处：《药学服务与研究》2020年第6期437-441,共5页Pharmaceutical Care and Research

基　　金：国家自然科学基金(81773991);上海市科学技术委员会科研计划项目(17401901300)。

摘　　要：线粒体功能障碍可导致细胞凋亡、坏死、代谢改变、肌无力、神经退行性病变、器官功能下降,并最终导致整个生物体疾病或死亡。线粒体毒性物质是导致细胞内线粒体数量减少或降低线粒体正常功能的化合物。高通量筛选(high throughput screening,HTS)技术在对线粒体毒性物质的检测应用方面具有巨大潜力。本文综述采用HTS方法体外测定线粒体毒性物质的研究进展,以期为线粒体毒性物质的筛选提供参考。Mitochondrial dysfunction can lead to apoptosis,necrosis,altered metabolism,muscle weakness,neurodegeneration and decreased organ function,thus resulting in disease or death of the whole organism.Mitochondrial toxicants are compounds that may cause decrease in the number of intracellular mitochondria or decrease in the normal function of mitochondria.High throughput screening(HTS)has great potential for the test and detection of toxic agents on mitochondria.This paper reviewed the research progress in the determination of mitochondrial toxicants by HTS technology in vitro,so as to provide reference for screening mitochondrial toxicants in vitro.

关 键 词：高通量筛选 线粒体毒性 体外评价 综述 

分 类 号：R994.39[医药卫生—毒理学]