基于核磁共振氢谱探讨复方缓溃乐混悬剂对溃疡性结肠炎大鼠血清代谢谱的影响  

Effect of compound Huankuile suspension on serum metabolic profile in rats with ulcerative colitis based on H nuclear magnetic resonance

作　　者：阿依加玛力·沙依提 卡思木江·阿西木江[1] 许晨波[1] 希林古丽·吾守尔 吴桂霞[1] 纳菲沙·卡德尔[1] 库热西·玉努斯 AYIJIAMALI·Sha-yi-ti;KASIMUJIANG·A-xi-mu-jiang;XU Chen-bo;XILINGULI·Wu-shou-er;WU Gui-xia;NAFEISHA·Ka-de-er;KUREXI·Yu-nu-si(School of Basic Medical Sciences,Xinjiang Medical University,Urumqi 830011,Xinjiang Uyghur Autonomous Region,China;Uyghur Medical College,Xinjiang Medical University,Urumqi 830011,Xinjiang Uyghur Autonomous Region,China)

机构地区：[1]新疆医科大学基础医学院,新疆维吾尔自治区乌鲁木齐830011 [2]新疆医科大学维吾尔医学院,新疆维吾尔自治区乌鲁木齐830011

出　　处：《中国临床药理学杂志》2020年第23期3910-3913,共4页The Chinese Journal of Clinical Pharmacology

基　　金：新疆维吾尔自治区“十三五”重点学科建设基金资助项目(高原学科)

摘　　要：目的探讨复方缓溃乐混悬剂(HKL)对溃疡性结肠炎(UC)大鼠紊乱代谢物的影响,并分析其治疗UC可能的作用机制。方法用2,4,6-三硝基苯磺酸(TNBS)/乙醇复合法建立UC模型;另选大鼠予以生理盐水灌肠作为正常组。按照随机数字表法将造模成功大鼠分为3组:阴性对照组、5-氨基水杨酸组(5-ASA组)和HKL组,每组12只;其中5-ASA组与HKL组大鼠分别以1 mL·200 g^-15-ASA和1 mL·100 g^-1 HKL灌胃,正常组和阴性对照组灌胃等量0.9%NaCl,均连续灌胃14 d。利用核磁共振氢谱(1H-NMR)技术结合正交偏最小二乘法-判别式分析(OPLS-DA)法筛选以上4组之间变量重要性投影值(VIP)>1的差异性代谢物。结果阴性对照组与正常组比较,阴性对照组大鼠血清中甘氨酸、乳酸、α-葡萄糖、丙酮酸和肉碱的VIP值分别为2.86,2.89,2.13,1.13和1.08。HKL组与阴性对照组比较,HKL组大鼠血清中α-葡萄糖、肉碱、牛磺酸、乳酸、极低密度脂蛋白(VLDL)的VIP值分别为5.74,1.04,5.44,7.10和2.75。与5-ASA组比较,HKL组中缬氨酸含量降低。结论HKL可调节UC大鼠所出现的代谢紊乱,其主要与调节氨基酸代谢、能量代谢及糖酵解途径有关。Objective To investigate the effect of compound Huankuile(HKL)on disordered metabolites of ulcerative colitis(UC)rats,and analyze its possible mechanism of treatment of UC.Methods The Wistar rats UC model were established by 2,4,6-trinitrobenzene sulfonic acid(TNBS)/ethanol,the other rats were randomly set as the normal group.After making the model successfully,rats were randomly divided into 3 groups:Negative control group,5-amino salicylic acid intervention group(5-ASA group)and HKL group,each group had 12 rats;among them,rats in the 5-ASA group and the HKL group were gaved with 1 mL·200 g^-15-ASA and 1 mL·100 g^-1 HKL,respectively.The normal group and the negative control group were given with an equal amount of normal saline,all were given intragastrically for 14 days.Differential metabolites with variable importance projection value(VIP)>1 between the above four groups were screened using nuclear magnetic resonance hydrogen spectroscopy(1H-NMR)technology combined with orthogonal partial least squares-discriminant analysis(OPLS-DA)method.Results Comparing the negative control group with the normal group,the VIP values of glycine,lactic acid,α-glucose,pyruvate,and carnitine in the serum of the negative control group were 2.86,2.89,2.13,1.13 and 1.08.Compared with the negative control group,the VIP values ofα-glucose,carnitine,taurine lactic acid and very low density lipoprotein(VLDL)in the serum of HKL group rats the VIP values were 5.74,1.04,5.44,7.10 and 2.75.Compared with the 5-ASA group,the valine content in the HKL group decreased.Conclusion HKL has a certain therapeutic effect in UC rats,and the mechanism may be related to regulating amino acid metabolism,energy metabolism,glycolysis.

关 键 词：复方缓溃乐混悬剂 溃疡性结肠炎 代谢组学 

分 类 号：R28[医药卫生—中药学]