丹参酮ⅡA对人胃癌细胞SGC7901抑制作用  

Inhibitory effect of tanshinoneⅡA on human gastric cancer cell line SGC7901

作　　者：刘红文[1] 刘琪 王贞 LIU Hong-wen;LIU Qi;WANG Zhen(Department of Pharmacy,Qinghai Red Cross Hospital,Xining 810000,Qinghai Province,China;Department of Gastroenterology,the Fifth People's Hospital of Qinghai Province,Xining 810000,Qinghai Province,China)

机构地区：[1]青海红十字医院药学部,青海西宁810000 [2]青海省第五人民医院消化内科,青海西宁810000

出　　处：《中国临床药理学杂志》2020年第23期3926-3929,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究丹参酮ⅡA(TanⅡA)对人胃癌细胞SGC7901抑制作用及其与环氧合酶-2(COX-2)表达及核因子-κB(NF-κB)信号通路的关系。方法SGC7901细胞分为实验组极低、低、中、高剂量和对照组,极低、低、中、高剂量实验组用0.5,1.0,2.0,4.0μg·mL^-1 TanⅡA干预,对照组无任何药物处理。培养24,48,72 h后,用噻唑蓝(MTT)法检测细胞增殖情况;以流式细胞仪检测细胞凋亡情况;以蛋白质印迹法检测细胞COX-2及NF-κB信号通路相关蛋白表达情况;以酶联免疫吸附(ELISA)法测定前列腺素E2(PGE2)水平。结果干预后72 h,极低、低、中、高剂量实验组增殖抑制率分别为(9.64±2.28)%,(25.09±1.88)%,(45.86±2.88)%,(55.68±3.22)%。培养72 h后,极低、低、中、高剂量实验组细胞凋亡率高于对照组,且随药物浓度的升高而增大(均P<0.05)。对照组与极低、低、中、高剂量实验组上清中PGE2分别为(115.46±6.58),(88.46±3.26),(65.16±3.21),(42.18±1.95),(39.47±1.52)ng·L^-1,差异有统计学意义(P<0.05)。实验组COX-2、p65、p-IκB-α、IKK-β、p-IKK-β蛋白表达量均低于对照组(均P<0.05),IκB-α、IKK-α及p-IKK-α蛋白表达量与对照组比较,差异无统计学意义(均P>0.05)。结论TanⅡA可抑制SGC7901细胞增殖,并促其凋亡,且呈剂量-时间依赖性,可能与降低COX-2及NF-κB通路相关蛋白水平,进而降低COX-2/PGE2及NF-κB通路信号转导活性相关。Objective To investigate the inhibitory effect of tanshinoneⅡA(TanⅡA)on human gastric cancer cell SGC7901 and its relationship with cyclooxygenase-2(COX-2)expression and nuclear factor-κB(NF-κB)signaling pathway.Methods SGC7901 cells were divided into very low,low,medium and high dose test groups and control group.Very low,low,medium and high dose test groups were treated with 0.5,1.0,2.0 and 4.0μg·mL^-1 TanⅡA,control group was given no drug treatment.After 24,48 and 72 h incubation,the proliferation was detected by MTT assay,the cell apoptosis was detected by flow cytometry,the expression of COX-2 and NF-κB signaling pathway related protein were detected by Western blotting(WB),the release of prostaglandin E2(PGE2)was detected by enzyme-linked immunosorbent assay(ELISA).Results After incubation for 72 h,the proliferation inhibition rate in very low,low,medium and high dose test groups were(9.64±2.28)%,(25.09±1.88)%,(45.86±2.88)%,(55.68±3.22)%.After incubation for 72 h,the apoptosis rate of very low,low,medium and high dose test groups were higher than that of control group,and which increased with the increasing of TanⅡA concentration(P<0.05).The PGE2 in cell culture supernatant of control group and very low,low,medium and high dose test groups were(115.46±6.58),(88.46±3.26),(65.16±3.21),(42.18±1.95),(39.47±1.52)ng·L^-1,all with significant difference(all P<0.05).The expression levels of COX-2,p65,p-IκB-α,IKK-βand p-IKK-βin test group were lower than those in control group(all P<0.05),there was no significant difference in the expression of IκB-α,IKK-αand p-IKK-α2 between two groups(all P>0.05).Conclusion TanⅡA can significantly inhibit the proliferation of gastric cancer cell line SGC7901 and promote its apoptosis in a dose-time dependent manner,and its mechanism may be related to the decrease of COX-2 level and NF-κB pathway signal transduction activity.

关 键 词：丹参酮ⅡA 胃癌 SGC7901细胞 抑制 环氧合酶-2 核因子-ΚB信号通路 

分 类 号：R28[医药卫生—中药学]