均腐土不同亚纲典型土系土壤细菌群落多样性研究  

Diversity of soil bacterial community in typical Isohumisols with different soil series

作　　者：焦峰[1] 吕淑敏 唐雷 张东萌 王光华[2] JIAO Feng;LV Shu-min;TANG Lei;ZHANG Dong-meng;WANG Guang-hua(Heilongjiang Bayi Agricultural University,Daqing Heilongjiang 163319;Key laboratory of Mollosols Agroecology,Northeast Institute of Geography and Agroecology,China Academy of Sciences,Harbin Heilongjiang 150081)

机构地区：[1]黑龙江八一农垦大学,黑龙江大庆163319 [2]中国科学院东北地理与农业生态研究所,黑土农业生态重点实验室,黑龙江哈尔滨150081

出　　处：《中国土壤与肥料》2020年第6期154-162,共9页Soil and Fertilizer Sciences in China

基　　金：黑龙江省自然科学基金项目(D2018005)。

摘　　要：为研究中国土壤系统分类(CST)体系下均腐土不同亚纲土壤细菌群落结构的多样性,以4个干润均腐土[富牧西系(DFm)、春雷南系(DCl)、保国系(DBg)、明水系(DMs)]和4个湿润均腐土[大西江系(MDx)、新发北系(MXf)、卫星农场系(MWx)、裴德系(MPd)]的腐殖质层(Ah层)为研究对象,采用高通量测序技术研究细菌群落多样性,分析细菌群落结构与环境因子间的关系,以探讨影响均腐土不同亚纲群落结构的主要环境因子。结果表明:均腐土8个土系样品共获得1 674 634条基因序列,Shannon指数和Chao1指数表现为:干润均腐土>湿润均腐土。均腐土8个土系细菌群落主要包括10个门类,其中变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)为富牧西系、春雷南系、保国系、大西江系、新发北系、卫星农场系、裴德系优势菌门,明水系以绿湾菌门(Chloroflexi)、变形菌门(Proteobacteria)、放线菌门(Actinobacteria)为优势菌门;属水平上,均腐土各土系的群落差异较大。样品热图分析将8个土系细菌群落聚为2类,其中干润均腐土聚为一类,湿润均腐土聚为一类。pH、交换性Ca、CEC、交换性Mg、SOC和TP是影响均腐土细菌群落结构发生变化的主要因子(P<0.05)。此外,RDA分析发现,土壤p H为影响均腐土细菌群落结构的主导因子,而pH、交换性Ca、CEC为驱动干润均腐土细菌群落结构发生变化的主要影响因素,交换性Mg、SOC、TP为驱动湿润均腐土细菌群落发生变化的主要影响因素。In order to study the diversity of Isohumisols bacterial community structure in different subclass of the Chinese soil system classification(CST)system,the humus layer(Ah)of the 4 Dry Isohumisols[Fumuxi Series(DFm),Chunleinan Series(DCl),Baoguo Series(DBg),Mingshui Series(DMs)]and 4 Moist Isohumisols[Daxijiang Series(MDx),Xinfabei Series(MXf),Weixingnongchang Series(MWx),Peide Series(MPd)]were used as the research objects.The high-throughput sequencing technology was used to study the diversity of bacterial communities,and the relationship between bacterial community structure and environmental factors was analyzed to explore the main environmental factors affecting the different sub-family community structures of homogeneous mulch.The results showed that the 1674634 gene sequences were obtained from the Isohumisols.The Shannon and Chao1 indexes showed as:Dry Isohumisols>Mosit Isohumisols.In this study,the 8 soil series of Isohumisols was mainly comprised of 10 phylum.The dominant bacterial phylum were the Proteobacteria,Actinobacteria and Acidobacteria in Fumuxi Series,Chunleinan Series,Baoguo Series,Daxijiang Series,Xinfabei Series,Weixingnongchang Series,Peide Series.The Chloroflexi,Proteobacteria,and Actinobacteria were the dominant populations in Mingshui Series.At the genus level,the composition of Isohumisols bacterial communities was different from each other.According to the sample heat map analysis,the bacterial communities of 8 soil systems were grouped into 2 groups,the Dry Isohumisols was grouped into 1 group,and the Moist Isohumisols was grouped into 1 group.pH,EX-Ca,CEC,EX-Mg,SOC and TP were the main factors affecting the changes of bacterial community structure in the Isohumisols(P<0.05).In addition,the RDA analysis found that soil pH was the leading factor affecting the community structure of Isohumisoils,while pH,EX-Ca and CEC were the main factors driving the change of the bacterial community structure of Dry Isohumisols,and EX-Mg,SOC and TP were the main factors driving the change of the bacte

关 键 词：均腐土 干润均腐土 湿润均腐土 细菌群落 高通量测序 多样性 

分 类 号：S154.3[农业科学—土壤学]