儿童感染后闭塞性细支气管炎早期外周血中淋巴细胞亚群分析  

Analysis of lymphocyte subsets in peripheral blood of children with early stage of post-infectious bronchiolitis obliterans

作　　者：李林瑞[1] 张喜[1] 陈艳萍[1] LI Linrui;ZHANG Xi;CHEN Yanping(Hunan Children's Hospital,Changsha 410006,China)

机构地区：[1]湖南省儿童医院呼吸内科,长沙410006

出　　处：《中国中西医结合儿科学》2020年第6期524-526,共3页Chinese Pediatrics of Integrated Traditional and Western Medicine

摘　　要：目的探讨外周血中淋巴细胞亚群变化对于早期识别儿童感染后闭塞性细支气管炎(BO)的意义。方法选取2016年6月至2018年6月在湖南省儿童医院住院的、入院初期诊断为重症肺炎的患儿48例,24例确定腺病毒感染后BO患儿为观察组,同期选取重症肺炎未进展为BO的患儿24例为对照组。两组患儿均在入院诊断为重症肺炎时采用流式细胞计量术测定淋巴细胞亚群。结果两组全血总T淋巴细胞及总B淋巴细胞数目及比值差异无统计学意义(P>0.05);观察组CD4细胞数及其比值均显著低于对照组,差异有统计学意义(P<0.05);两组CD8细胞数比较差异无统计学意义(P>0.05),但是观察组CD8细胞比值高于对照组,差异有统计学意义(P<0.05);观察组自然杀伤(NK)细胞数及其比值低于对照组,差异有统计学意义(P<0.05);两组CD4/CD8比值比较,差异有统计学意义(P<0.05)。结论继发感染后BO的重症肺炎患儿CD4,NK细胞数及比值可能作为早期识别BO的指标。Objective To investigate the significance of lymphocyte subsets changes in peripheral blood for early identification of children with post-infectious bronchiolitis obliterans(BO).Methods Forty-eight children admitted to Hunan Children's Hospital from June 2016 to June 2018,who were diagnosed with severe pneumonia at the initial stage of admission,were selected;the 24 cases of BO confirmed after adenovirus infection were in the observation group,and 24 children with severe pneumonia that did not progress to BO in the same period were selected as the control group.The lymphocyte subsets in both groups were determined by flow cytometry upon admission diagnosis of severe pneumonia.Results There was no significant difference in the number or ratio of total T lymphocytes and total B lymphocytes between the two groups(P<0.05).The number and ratio of CD4 cells in the observation group were significantly lower than those in the control group,and the difference was statistically significant(P<0.05).There was no statistically significant difference in the number of CD8 cells between the two groups(P<0.05),but the ratio of CD8 cells in the observation group was higher than that in the control group(P<0.05).The number and ratio of natural killer(NK)cells in the observation group were lower than those in the control group,and the difference was statistically significant(P<0.05).The difference in CD4/CD8 ratio between the two groups was statistically significant(P<0.05).Conclusion The number and ratio of CD4 and NK cells in children with severe pneumonia of secondary post-infectious BO may be used as indicators for early identification of BO.

关 键 词：闭塞性细支气管炎 重症肺炎 CD4细胞 CD8细胞 NK细胞 

分 类 号：R725.6[医药卫生—儿科]