复方沙太合剂抑制IL-17a的表达改善类风湿关节炎小鼠脾脏病理损伤的作用研究  

Inhibition of Fufang Shatai mixture on the expression of IL-17a and improving the pathological damage of spleen in collagen-induced arthritis mice

作　　者：范凯健 吴菁[1] 许冰馨 王琪珊 王婷玉[1] 顾方[1] FAN Kai-jian;WU Jing;XU Bing-xin;WANG Qi-shan;WANG Ting-yu;GU Fang(Department of Pharmacy,Shanghai Ninth People’s Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200011;Department of Pharmacy,Mental Health Center,Chongming District,Shanghai 202150)

机构地区：[1]上海交通大学医学院附属第九人民医院药剂科,上海200011 [2]上海市崇明区精神卫生中心药剂科,上海202150

出　　处：《中南药学》2020年第12期1992-1995,共4页Central South Pharmacy

基　　金：国家自然科学基金(No.81874011);上海市科委科研项目(No.18140903502)。

摘　　要：目的研究复方沙太合剂(STM)对类风湿关节炎小鼠脾脏免疫功能的调节作用。方法将30只DBA/1J小鼠随机分为3组,分别为正常组、模型组和给药组。模型组和给药组建立Ⅱ型胶原诱导的类风湿关节炎(CIA)模型。造模成功后,STM给药组给予STM 10 mL·kg-1灌胃治疗,连续5周。正常组和模型组用等量生理盐水进行处理。给药结束后,处死各组小鼠,收集血清、脾脏,对脾脏进行病理和免疫组化分析。酶联免疫吸附测定(ELISA)法检测小鼠外周血中白细胞介素17a(IL-17a)的表达情况。结果与模型组相比,在给予STM治疗后,小鼠关节炎症状明显减轻。脾脏HE染色显示,STM可以抑制红髓增殖,增厚脾脏包围的纤维囊,减少铁血色颗粒物的出现。脾脏免疫组化结果显示,STM可以抑制脾脏淋巴细胞Ki-67、IL-17a的免疫阳性表达。ELISA结果显示,STM可以显著抑制IL-17a的表达。结论 STM可以通过改善CIA小鼠的脾脏结构、抑制脾淋巴细胞的增殖和T淋巴细胞相关炎症因子IL-17a的表达来发挥免疫调节作用。Objective To determine the regulation of Fufang Shatai mixture (STM) on the immune function of the spleen in mice with rheumatoid arthritis.Methods Thirty DBA/1J mice were randomly divided into three groups:a normal group,a model group and an STM group.The collagen-induced arthritis (CIA) model was established in the model and the STM group by type Ⅱ collagen.After successful modeling,STM (10 mL·kg-1) was given intragastrically for 5 consecutive weeks in the STM group.The normal and the model groups were treated with normal saline.After the administration,the mice in each group were sacrificed,the serum and the spleen were collected,and the spleen was analyzed by pathology and immunohistochemistry.Enzyme linked immunosorbent assay (ELISA) was used to detect the expression of interleukin 17a (IL-17a) in the peripheral blood of mice.Results Compared with the model group,after the STM treatment,arthritis symptoms were significantly reduced.HE staining of the spleen showed that STM inhibited the proliferation of red pulp,thickened the fibrous capsule surrounded by the spleen,and reduced the appearance of iron-colored particles.Spleen immunohistochemistry showed that STM inhibited the immunopositive expression of Ki-67 and IL-17a in the spleen lymphocytes.ELISA showed that STM significantly inhibited the expression of IL-17a.Conclusion STM exerts immunoregulatory effects by improving the spleen structure in CIA mice,inhibiting the proliferation of the splenic lymphocytes and the expression of IL-17a,an inflammatory factor related to T lymphocytes.

关 键 词：胶原诱导性关节炎 复方沙太合剂 脾脏 免疫 

分 类 号：R285[医药卫生—中药学]