日粮中添加NCG对妊娠前期鄂尔多斯细毛羊血液、尿囊液和羊水中氨基酸浓度的影响  

Effects of Different NCG Levels in Diet on Amino Acids Concentrations in Plasma,Allantoic Fluid,Amniotic Fluid during Pre-pregnancy of Ordos Fine Wool Sheep

作　　者：吴宝升 张崇志 孙海洲 桑丹 李胜利 张春华 金鹿 斯登丹巴 谷英 WU Baosheng;ZHANG Chongzhi;SUN Haizhou;SANG Dan;LI Shengli;ZHANG Chunhua;JIN Lu;Sidengdanba;GU Ying(Institute of Animal Nutrition and Feed,Inner Mongolia Academy of Agricultural&Animal Husbandry Sciences,Inner Mongolia Key Laboratorg of Nutrition Science of Herbivore Hohhot,Inner Mongolia 010031,China;Ordos Academy of Agricultural&Animal Husbandry Sciences,Erdos,Inner Mongolia 017000,China)

机构地区：[1]内蒙古自治区农牧业科学院内蒙古自治区草食动物营养科学重点实验室,内蒙古呼和浩特010031 [2]鄂尔多斯市农牧业科学研究院,内蒙古鄂尔多斯017000

出　　处：《家畜生态学报》2021年第1期37-45,共9页Journal of Domestic Animal Ecology

基　　金：国家公益性行业(农业)科研专项(201303062);国家现代农业产业技术体系建设专项(CARS-39-11);内蒙古自治区科技创新引导项目;内蒙古自治区创新人才团队项目;工程培养(引进)高层次人才项目;引进国际先进农业科学技术“948”(2014-Z41)。

摘　　要：为了研究日粮中添加NCG(N-氨甲酰谷氨酸)对妊娠前期鄂尔多斯细毛羊血液、尿囊液和羊水中氨基酸浓度的影响,试验选择年龄、胎次和体况相近、平均体重为50.62±0.52 kg的受孕鄂尔多斯细毛羊母羊40只,随机分为3组(NCG1组饲喂基础日粮+0.30 g NCG/d(n=13),NCG2组饲喂基础日粮+0.40 g NCG/d(n=13),对照组饲喂基础日粮(n=14)),开展妊娠期0 d至90 d的饲喂试验。结果表明,日粮添加不同剂量的NCG对妊娠前期鄂尔多斯细毛羊体重、胎儿体重、尿囊液和羊水含量没有显著影响(P>0.05)。妊娠期第45 d,NCG2组母羊血浆中瓜氨酸和鸟氨酸浓度显著高于NCG1组(P<0.05)和对照组(P<0.01);NCG1组和NCG2组母羊尿囊液中谷氨酰胺浓度显著高于对照组(P<0.05)。妊娠期第90 d,NCG2组母羊血浆中丝氨酸和精氨酸(Arg)浓度显著高于对照组(P<0.05);NCG2组和NCG1组母羊尿囊液中Arg浓度显著高于对照组(P<0.05),NCG1组鸟氨酸浓度显著高于NCG2组(P<0.05)和对照组(P<0.01);NCG2组母羊羊水中谷氨酰胺、Arg和瓜氨酸浓度显著高于对照组(P<0.05),NCG2组和NCG1组鸟氨酸浓度显著高于对照组(P<0.05);NCG2组和NCG1组胎儿血浆中苏氨酸和鸟氨酸浓度极显著高于对照组(P<0.01),NCG2组和NCG1组Arg浓度显著高于对照组(P<0.05)。妊娠前期NCG有效提高母羊血浆、尿囊液、羊水和胎儿血浆中Arg家族氨基酸的浓度,对胎儿早期程序化的健康生长发挥重要作用。This study investigated the effects of different NCG levels in diet on amino acids concentrations in plasma,allantoic fluid and amniotic fluid during pre-pregnancy of Ordos fine wool sheep.Forty healthy Ordos fine wool sheep during pregnancy with similar body weight(50.62±0.52)kg,age and parity were randomly allocated into 3 groups(NCG1 group fed basal diet+0.30 g NCG/d(n=13),NCG2 group fed basal diet+0.40 g NCG/d(n=13),and control group fed basal diet(n=14).The experiment lasted from 0 day to 90 days of gestation.The results showed that there were no significant changes on the maternal body weight,fetal body weight,allantoic fluid and amniotic fluid during pre-pregnancy of Ordos fine wool sheep(P>0.05).At 45 days of gestation,the Cit and Orn concentrations in maternal plasma of NCG2 group significantly increased compared to NCG1 group(P<0.05)and control group(P<0.01);the Gln concentration in allantoic fluid of NCG1 and NCG2 groups was significantly increased compared to control group(P<0.05).At 90 days of gestation,the Ser and Arg concentrations in maternal plasma of NCG2 group were significantly higher than in control group(P<0.05),and the Arg concentration in allantoic fluid of NCG2 and NCG1 groups was significantly higher than in control group(P<0.05),and the Orn concentration in NCG1 group was significantly higher than in NCG2 group and control group(P<0.05);the Gln,Arg and Cit concentrations in amniotic fluid of control group were significantly higher compared to NCG2 group(P<0.05),and the Orn concentration in control group was higher compared to NCG2 and NCG1 groups(P<0.05);the Thr and Orn concentrations in fetal plasma of NCG2 and NCG1 groups were highly significantly increased compared to control(P<0.01),and the Arg concentration in NCG2 and NCG1 groups was significantly increased compared to control group(P<0.05).In the experimental conditions,the addition of NCG in diet during pre-pregnancy effectively promoted the Arg family concentrations in maternal plasma,allantoic fluid,amniotic fluid and fetal p

关 键 词：妊娠前期 细毛羊 N-氨甲酰谷氨酸 精氨酸 

分 类 号：S811.5[农业科学—畜牧学]