黄连碱对脑胶质瘤U373细胞的增殖抑制作用研究  

A Study on the Inhibitory Effects of Coptisine on the Proliferation of Gliomas U373 Cells

作　　者：韦晟[1] 刘金春[1] 葛卫红[1] WEI Sheng;LIU Jinchun;GE Weihong(Nanjing Drum Tower Hospital,The Affiliated Hospital of Nanjing University Medical School,Narying 210008,China)

机构地区：[1]南京大学医学院附属鼓楼医院,江苏南京210008

出　　处：《西部中医药》2020年第10期22-25,共4页Western Journal of Traditional Chinese Medicine

基　　金：江苏省医疗保险研究会项目(JSYB2017002)。

摘　　要：目的:探讨黄连碱对人脑胶质瘤U373细胞的增殖抑制作用。方法:使用不同浓度的黄连碱处理体外培养的脑胶质瘤细胞系U373后,应用MTS法检测黄连碱对U373细胞增殖的影响;应用流式细胞仪检测黄连碱对U373细胞凋亡和周期的影响。结果:黄连碱对U373细胞表现出明显的增殖抑制作用,作用24、48和72 h后的IC50值分别是59.05、24.79和10.62μmol/L。黄连碱对U373细胞的凋亡没有明显的影响,黄连碱能够诱导U373细胞周期阻滞于G0/G1期,并表现出良好的浓度依赖性。结论:黄连碱对U373细胞具有显著的增殖抑制作用,并具有时间和剂量依赖性,且黄连碱是通过将细胞周期阻滞在G0/G1期进而非诱导U373细胞凋亡抑制U373细胞增殖的,其具体作用机制有待进一步研究。Objective: To explore the inhibitory effects of coptisine on the proliferation of human gliomas U373 cells. Methods: Different concentrations of coptisine were used to deal with human gliomas U373 cells cultured in vitro, MTS method was used to detect the effects of coptisine on the proliferation of U373 cells;flow cytometry was applied to detect its influence on the apoptosis and the cycle of U373 cells. Results: Coptisine showed notable inhibitory effects on the proliferation of U373 cells, IC50 values after acting on for 24, 48 and 72 hours were59.05, 24.79 and 10.62 μmol/L respectively. Coptisine showed no obvious influence on the apoptosis of U373 cells,it could induce U373 cells cycle arrest in G0/G1 phase, showing in a good concentration-dependent manner.Conclusion: Coptisine possess the significant proliferation-inhibition effects on U373 cells, in a time-and dosedependent manners, and it could inhibit the proliferation of U373 cells through inducing cellular cycle arrest in G0/G1 phase, while not inducing the apoptosis of U373 cells, and its specific mechanism needs further study.

关 键 词：黄连碱 U373细胞 细胞增殖 细胞周期 

分 类 号：R285[医药卫生—中药学]