PI3K/Akt信号通路在小檗碱联合人参皂苷Rg3诱导鼻咽癌细胞凋亡中的调控作用  

The regulation role of PI3K/Akt signaling pathway in berberine combined with ginsenoside Rg3 induced apoptosis of nasopharyngeal carcinoma cells

作　　者：周芳亮[1,2,3] 胡晶 蔺婷[1,4] 何兰[1,4] 范婧莹[1,4] 罗晶婧[1,3,4] 王贤文 何迎春[1,2,3,4] 廖端芳[2] ZHOU Fang-liang;HU Jing;LIN Ting;HE Lan;FAN Jing-ying;LUO Jing-jing;WANG Xian-wen;HE Ying-chun;LIAO Duan-fang(Hunan Provincial Key Lab for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine,Hunan University of Chinese Medicine;Key Lab for Quality Evaluation of Bulk Herbs of Hunan Province State,Hunan University of Chinese Medicine;College of Medicine,Hunan University of Chinese Medicine;Hunan Provincial Engineering and Technological Research Center for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine and Protecting Visual Function,Changsha 410208,China)

机构地区：[1]湖南中医药大学中医药防治眼耳鼻咽喉疾病湖南省重点实验室 [2]湖南中医药大学湘产大宗药材品质评价湖南省重点实验室 [3]湖南中医药大学医学院 [4]湖南中医药大学湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心,湖南长沙410208

出　　处：《中国药理学通报》2021年第1期43-52,共10页Chinese Pharmacological Bulletin

基　　金：湖南省教育厅优秀青年基金(No 19B439);国家自然科学基金资助项目(No 81874408,81973914);湖南省自然科学基金资助项目(No 2019JJ40216,2020JJ5419);湖南省研究生创新项目(No CX2018B482);中医药防治眼耳鼻咽喉疾病湖南省重点实验室开放基金课题(No 2018YZD04),湖南中医药大学基础医学一流学科开放基金项目(No 2018JCYX08)。

摘　　要：目的研究PI3K/Akt信号通路在小檗碱联合人参皂苷Rg3诱导鼻咽癌细胞凋亡中的作用。方法RTCA法检测小檗碱联合人参皂苷Rg3对鼻咽癌CNE2、6-10B细胞增殖的影响;Hoechst 33342染色法、荧光双染流式细胞仪检测药物对CNE2细胞凋亡的影响;Western blot法检测PI3K/Akt信号通路关键蛋白及凋亡相关蛋白表达的变化。结果小檗碱联合人参皂苷Rg3可抑制鼻咽癌CNE2、6-10B细胞的增殖,诱导CNE2细胞的凋亡。与单独用药组相比,联合用药组细胞的PI3K/Akt信号通路关键蛋白PI3K p110α和p-Akt的表达明显下调(P<0.05)。加入PI3K/Akt信号通路的激活剂SC79后,小檗碱联合人参皂苷Rg3抑制CNE2细胞增殖和诱导细胞凋亡的效应降低,p-Akt、Survivin、PCNA及Bcl-2表达量增加,Bax的表达下降。结论小檗碱联合人参皂苷Rg3可通过PI3K/Akt信号通路发挥抑制鼻咽癌细胞增殖和诱导细胞凋亡的作用。Aim To study the effect of berberine combined with ginsenoside Rg3 on the apoptosis of nasopharyngeal carcinoma(NPC)cells,and to discuss the role of the PI3K/Akt signaling pathway in this process.Methods Real time cellular analysis(RTCA),fluo-rescence double-staining flow cytometry and Hoechst 33342 staining were used to detect the effects of berberine combined with ginsenoside Rg3 on the proliferation and apoptosis of NPC cells.Western blot was used to examine the effects of drugs on the expressions of apoptosis-related proteins and the key proteins of PI3K/Akt signaling pathway.Results Berberine combined with ginsenoside Rg3 inhibited the proliferation and induced cell apoptosis of NPC cells.Expressions of PI3K p110αand p-Akt were significantly down-regulated in combined drug group.After activation of PI3K/Akt signaling pathway,the effect of berberine combined with ginsenoside Rg3 on inhibiting CNE2 cell proliferation and inducing apoptosis was reduced.Compared with combination group,the levels of Survivin,PCNA and Bcl-2 were relatively enhanced,while the level of Bax declined(P<0.05).Conclusions Berberine combined with ginsenoside Rg3 may play a role in inhibiting the proliferation and inducing apoptosis of NPC cells through PI3K/Akt signaling pathway.

关 键 词：小檗碱 人参皂苷RG3 联合作用 细胞凋亡 鼻咽癌 PI3K/AKT信号通路 

分 类 号：R284.1[医药卫生—中药学] R285.5[医药卫生—中医学]