直接抗病毒药对慢性丙型肝炎患者外周血单个核细胞频率及其活化因子sCD14s、CD163的影响  

Effects of direct antiviral agent on the frequency of peripheral blood mononuclear cells and their activating factors sCD14s and CD163 in patients with chronic hepatitis C

作　　者：李建国[1] 曾国芬 曾映夫 李舣婷 宁钢[1] 林潮双[1] 张晓红[1] 高志良[1] Li Jianguo;Zeng Guofen;Zeng Tingfu;Li Yiting;Ning Gang;Lin Chaoshuang;Zhang Xiaohong;Gao Zhiliang(Department of Infectious Diseases,The Third Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510630,China)

机构地区：[1]中山大学附属第三医院感染科,广州510630

出　　处：《中华肝脏病杂志》2020年第12期1018-1022,共5页Chinese Journal of Hepatology

基　　金：广东省省级科技计划项目(2014B02022025)。

摘　　要：目的探索直接抗病毒药(DAAs)对慢性丙型肝炎患者外周血单个核细胞频率及其活化因子sCD14s、CD163的影响。方法收集初治慢性丙型肝炎患者15例,健康对照者10名资料﹔慢性丙型肝炎患者给予DAAs治疗12周,分别在第0,4、12周收取血标本,健康对照者血标本作为对照。流式细胞术检测外周血经典型CD14^+CD16^+单个核细胞和促炎型CD14CD16^+单个核细胞频率,用酶联免疫吸附法检测血清sCD14s、sCD163。计量资料两组间比较采用t检验,多组间比较先行方差分析,进一步两两比较,用LSD-t检验。结果DAAs治疗前外周血CD14^+CD16^+单个核细胞频率(18.49%±1.54%比10.65%±0.83%)、血清sCD14s[(64407.38±5778.49)pg/ml比(28370.76±2357.68)pg/ml]和sCD163[(22853.80±4137.61)pg/ml比(2934.41±223.31)pg/ml]均高于健康对照者(P<0.05),而外周血CD14^+CD16^+单个核细胞频率则低于健康对照者(59.14%±0.54%比72.75%±1.31%,P<0.01)。DAAs治疗过程中CD14^+CD16^+单个核细胞频率、血清sCD14和sCD163均显著下降,治疗12周后CD14^+CD16^+单个核细胞降至接近正常水平(12.42%±1.60%比10.65%士0.83%,P>0.05),血清sCD14和sCD163仍高于健康对照者[sCD14:(44390.06±3330.17)pg/ml比(28370.76±2357.68)pg/ml,P<0.01,sCD163:(11494.79±1836.97)pg/ml比(2934.41±223.31)pg/ml,P<0.01],而CD14^+CD16^+单个核细胞频率DAAs治疗过程中逐渐升高,并在治疗12周后接近健康对照水平,两组差异无统计学意义(71.54%±2.99%比72.75%±1.31%,P>0.05)。结论DAAs治疗可降低慢性丙型肝炎患者外周血单个核细胞的活化。Objective To explore the effects of direct antiviral agent(DAAs)on the frequency of peripheral blood mononuclear cells and their activating factors sCD14s and CD163 in patients with chronic hepatitis C.Methods Data of 15 treatment-naive chronic hepatitis C patients and 10 healthy controls were collected.Patients with chronic hepatitis C were treated with DAAs for 12 wecks.Blood samples were collected at 0,4 and 12 weeks respectively,and blood samples of healthy controls were used as controls.Flow cytometry was used to detect the frequency of classical CD14^+CD16^+ mononuclear cells and pro-inflammatory CD14^+CD16^+ mononuclear cells in peripheral blood.Serum sCD14s and sCD163 were detected by enzyme-linked immunosorbent assay.The comparison between the two groups was performed by t-test.The comparison between multiple groups was performed by analysis of variance,and further pairwise comparison was performed by LSD-t test.Results Prior DAAs treatment,peripheral blood CD14^+CD16^+mononuclear cell frequency(18.49%±1.54%vs.10.65%±0.83%),serum sCD14s[(64407.38±5778.49)pg/ml vs.(28370.76±2357.68)pg/ml]and sCD163[(22853.80±4137.61)pg/ml vs.(2934.41±223.31)pg/ml]were all higher than healthy controls(P<0.05),while the frequency of CD14^+CD16^+ mononuclear cells inperipheral blood was lower than healthy controls(59.14%+0.54%vs.72.75%=1.31%,P<0.01).During DAAstreatment,CD14^+CD16^+ mononuclear cells frequency,serum sCD14 and sCD163 wcre all decreased significantly.After 12 weeks of treatment,CD14^+CD16^+ mononuclear cells had decreased to nearly normal level(12.42%±1.60%vs.10.65%±0.83%,P>0.05),and serum sCD14 and scd163 were still higher than those of healthy controls[sCD14:(44390.06±3330.17)pg/ml vs.(28370.76±2357.68)pg/ml,Scd163:(11494.79±1836.97)pg/ml vs.(2934.41±223.31)pg/ml,P<0.01].while the frequency of CD14^+CD16^+ mononuclear cells had graduallyincreased during the course of treatment and neared healthy control level after 12 weeks of treatment.There wasno statistically significant difference between the

关 键 词：肝炎病毒 丙型 单核细胞 直接抗病毒药 

分 类 号：R512.63[医药卫生—内科学]