2018年珠海市HIV-1基因型及耐药分析  

Genotypes and drug resistance of HIV-1 in Zhuhai, 2018

作　　者：周兰兰[1] 魏泉德[1] 黄辉涛 李晓凤[1] ZHOU Lan-lan;WEI Quan-de;HUANG Hui-tao;LI Xiao-feng(Zhuhai Municipal Center for Disease Control and Prevention,Zhuhai,Guangdong 519000,China)

机构地区：[1]珠海市疾病预防控制中心,广东珠海519000

出　　处：《实用预防医学》2020年第12期1409-1412,共4页Practical Preventive Medicine

基　　金：珠海市科技计划医疗卫生项目(ZH2202200071HJL)。

摘　　要：目的了解2018年广东省珠海市新确诊且未接受抗病毒治疗的HIV-1抗体阳性病例不同感染途径病毒亚型分布和耐药情况。方法收集研究对象血浆标本207份,提取病毒RNA,采用巢氏RT-PCR扩增HIV-1多聚酶pol区,测序定型和耐药分析。结果在男男性行为者中流行基因型为CRF07_BC(43.3%)、CRF01_AE(36.1%)和CRF55_01B(13.4%);在异性性感染人群优势基因型为CRF01_AE(52.5%)、CRF07_BC(31.1%)和CRF08_BC(6.6%);在3例注射吸毒感染人群检出CRF01_AE;有1例血源性感染病例检测基因型为H亚型;在MSM和异性性感染人群产生低度耐药,低度耐药率分别为4.1%和4.9%;在注射吸毒和血源性传播感染人群未检出耐药病例。结论在经性传播途径感染HIV-1多种基因型共同流行,以CRF01_AE和CRF07_BC基因型为主;在四类人群未检出低度以上耐药病例,总体传播性耐药呈低度流行。Objective To study the genotype distribution and drug resistance of newly-confirmed HIV-1 positive cases without antiretroviral therapy(ART) through different pathways of infection in Zhuhai, Guangdong province in 2018. Methods Two hundred and seven plasma samples were collected from the research subjects. Viral RNA was extracted, and HIV-1 pol gene was amplified by nested RT-PCR and sequenced. According to pol gene sequences, the subtype distribution and drug resistance of HIV-1 were analyzed. Results The epidemic genotypes in MSM were CRF07_BC(43.3%), CRF01_AE(36.1%)and CRF55_01B(13.4%), and those in heterosexually-acquired HIV infection cases were CRF01_AE(52.5%), CRF07_BC(31.1%) and CRF08_BC(6.6%). CRF01_AE was detected in three injecting drug users, and H subtype in one case of blood-borne infection. HIV-1 infection in MSM and heterosexually-acquired HIV infection cases showed low drug resistance, with the low drug resistance rates of 4.1% and 4.9%, respectively. No drug resistance case was detected in the injecting drug users and the blood-borne infection case. Conclusions CRF01_AE and CRF07_BC were predominant in sexually transmitted HIV-1 infections, and many other genotypes were prevalent together. Intermediate and high-level resistance cases were not detected in the above-mentioned four kinds of populations. Transmissible drug resistance rate was generally low in Zhuhai.

关 键 词：HIV-1 感染途径 基因型 亚型 耐药 

分 类 号：R512.91[医药卫生—内科学]