芦荟、番泻叶单味及配伍西洋参通便功效评价  

Evaluation on laxative efficacy of aloe, senna alone and in combination with Panax quinquefolium

作　　者：李娜[1] 陈叙汐 岳午阳 彭子豪 吴瑞 张立实[1] 陈锦瑶[1] LI Na;CHEN Xu-xi;YUE Wu-yang;PENG Zi-hao;WU Rui;ZHANG Li-shi;CHEN Jin-yao(Sichuan Provincial Key Laboratory for Food Safety Monitoring and Risk Assessment,West China School of Public Health,Sichuan University,Chengdu,Sichuan 610041,China)

机构地区：[1]四川大学华西公共卫生学院,四川省食品安全监测与风险评估重点实验室,四川成都610041

出　　处：《实用预防医学》2020年第12期1425-1429,共5页Practical Preventive Medicine

基　　金：国家高技术研究发展计划(863计划)基金(NO.2010AA023001)。

摘　　要：目的探究库拉索芦荟全叶冻干粉、番泻叶提取物单味及与西洋参提取物联合用药对便秘小鼠的通便功效及其作用机制。方法采用昆明种雄性小鼠180只,随机分为9组,包括:空白组、模型组、芦荟10倍组(L1组)、芦荟20倍组(L2组)、番泻叶10倍组(F1组)、芦荟10倍+西洋参10倍组(L1X1组)、芦荟20倍+西洋参20倍组(L2X2组)、番泻叶10倍+西洋参10倍组(F1X1组)、番泻叶20倍+西洋参20倍组(F2X2组),给药14 d后,通过灌胃盐酸洛哌丁胺悬液(6 mg/kg)诱导小鼠便秘模型。观察小鼠首粒黑便时间、6 h黑便粒数及小肠墨汁推进率,以判断受试物通便功效;并测定血清乙酰胆碱酯酶(acetylcholinesterase,AchE)含量、小肠组织内胶质细胞源性神经营养因子(glial cell-derived neurotrophic factor,GDNF)的mRNA表达,以探讨其作用机制。结果 0 d(F=1.627,P=1.121)、7 d(F=1.588,P=0.132)、14 d(F=1.571,P=0.137)的各组小鼠体重无明显差异。与模型组相比,各剂量组首粒黑便时间缩短,6 h黑便粒数增多。各组小肠总长度间无明显差异(F=0.424,P=0.903),各组墨汁推进长度(F=9.857,P<0.001)和墨汁推进率(F=10.489,P<0.001)间差异有统计学意义;与模型组相比,L2组、L1X1组、L2X2组及F2X2组墨汁推进长度与墨汁推进率显著增加(P<0.05);与L1组相比,L1X1组墨汁推进长度与墨汁推进率显著增加(P<0.05)。各组血清AchE含量间差异有统计学意义(F=2.447,P=0.016);与模型组相比,L2X2组、F1X1组血清AchE含量显著升高(P<0.05)。各组小肠组织内GDNF的mRNA表达量间差异有统计学意义(F=101.158,P<0.001);与模型组相比, L1组、L2组、L2X2组及F2X2组小鼠小肠组织内GDNF的mRNA表达量显著上调(P<0.05)。结论 L2组、L1X1组、L2X2组、F2X2组具有良好通便效应,且两种药物的联合用药效果更佳,其作用机制可能与促进AchE释放及增加小肠组织内GDNF表达有关。Objective To explore the laxative efficacy and mechanism of aloe vera freeze-dried powder, senna extract alone and in combination with Panax quinquefolium extract in mice with constipation. Methods One hundred and eighty male Kunming mice were randomly divided into 9 groups, including the blank group, the model group, the 10-fold aloe group(the L1 group), the 20-fold aloe group(the L2 group), the 10-fold senna group(the F1 group), the 10-fold aloe + 10-fold Panax quinquefolium group(the L1 X1 group), the 20-fold aloe + 20-fold Panax quinquefolium group(the L2 X2 group), the 10-fold senna + 10-fold Panax quinquefolium group(the F1 X1 group) and the 20-fold senna + 20-fold Panax quinquefolium group(the F2 X2 group). After 14 days of treatment, constipation mouse models were established by intragastric administration of 6 mg/kg loperamide hydrochloride suspension. Start time of the first black feces defecation, the amount of black feces within 6 hours and gastrointestinal transit ratio were observed to evaluate the effects of the laxatives tested on constipation. The serum levels of acetylcholinesterase(AchE) and the mRNA expression of glial cell-derived neurotrophic factor(GDNF) in small intestines were measured to explore the mechanism of action. Results There was no significant difference in body weight on 0 d(F=1.627, P=1.121), 7 d(F=1.588, P=0.132) and 14 d(F=1.571, P=0.137) among the groups. As compared with the model group, start time of the first black feces defecation was shortened and the amount of black feces within 6 hours increased in the dose groups. There was no significant difference in the total length of small intestine among the groups( F = 0. 424,P = 0. 903),but statistically significant differences were found in the length of ink propulsion( F = 9.857,P<0.001) and the rate of ink propulsion( F= 10.489,P<0.001) among the groups. As compared with the model group,the length of ink propulsion and the rate of ink propulsion in the L2 group,the L1 X1 group,the L2 X2 group and the F2 X2 group significa

关 键 词：库拉索芦荟 番泻叶 西洋参 便秘 小鼠 

分 类 号：R975[医药卫生—药品]