宁波市新型冠状病毒肺炎确诊病例SARS-CoV-2靶标基因水平及影响因素分析  

Concentration of target genes of SARS-CoV-2 and influencing factors in the confirmed cases with COVID-19 in Ningbo

作　　者：张丹丹[1] 傅小红[1] 焦素黎[1] 顾文珍[1] 许国章[1] ZHANG Dandan;FU Xiaohong;JIAO Suli;GU Wenzhen;XU Guozhang(Ningbo Center for Disease Control and Prevention,Ningbo,Zhejiang 315010,China)

机构地区：[1]宁波市疾病预防控制中心,浙江宁波315010

出　　处：《预防医学》2020年第12期1213-1216,共4页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：宁波市科技重大专项-新型冠状病毒肺炎疫情防控应急科技攻关项目(2020C50001);宁波市医疗卫生品牌学科(PPXK2018-10);浙江省医学重点学科(07-013)。

摘　　要：目的分析宁波市新型冠状病毒肺炎(COVID-19)病例确诊时严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)靶标基因水平及影响因素。方法通过中国疾病预防控制信息系统收集2020年1月21日—2月20日宁波市报告的COVID-19确诊病例人口学信息和临床资料,通过宁波市疾病预防控制中心和定点医院收集确诊时SARS-CoV-2ORF1ab基因和N基因的Ct值;比较不同性别、年龄、症状严重程度、病例类型、病例来源、采样时间和采样部位的Ct值。结果收集157例COVID-19确诊病例资料,40~<60岁67例,占42.68%。其中男性56例,占35.67%;女性101例,占64.33%。女性病例ORF1ab基因和N基因Ct值分别为29.96±5.28和30.38±4.90,均高于男性病例的27.56±4.94和28.03±4.88 (P<0.05)。不同采样时间ORF1ab基因和N基因的Ct值差异有统计学意义(P<0.05),发病后1~7 d采样检测的ORF1ab基因和N基因的Ct值均最低,分别为27.84±4.80和28.35±4.65。ORF1ab基因(rs=0.288,P=0.001)和N基因(rs=0.296,P=0.001) Ct值均与采样时间呈正相关。咽拭子和痰液标本检测的ORF1ab基因Ct值分别为29.19±4.85和28.74±6.40,N基因Ct值分别为29.61±4.60和29.22±6.10,差异均无统计学意义(P>0.05)。结论采样时间对SARS-CoV-2核酸ORF1ab基因和N基因的Ct值影响较大。Objective To learn the concentration of target genes of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) and the influencing factors in the confirmed cases with coronavirus disease 2019(COVID-19) in Ningbo.Methods Demographic information and clinical diagnosis of COVID-19 cases reported in Ningbo from January 21 to February 20, 2020 were collected through China Disease Control and Prevention Information System. The Ct values of ORF1 ab and N of the cases were collected through Ningbo Center for Disease Control and Prevention and designated hospitals, and analyzed in terms of gender, age, severity, clinical classification, source of case, sampling time and specimen type. Results There were 157 confirmed COVID-19 cases, including 56 males( 35.67%) and 101 females( 64.33%).Sixty-seven cases( 42.68%) aged between 40 and 60 years old. The Ct values of ORF1 ab and N in females were 29.96±5.28 and 30.38±4.90, which were higher than 27.56±4.94 and 28.03±4.88 in males( P<0.05). The Ct values of ORF1 ab and N were the lowest when sampled at 1-7 days after onset( P<0.05), which were 27.84±4.80 and 28.35±4.65. The Ct values of ORF1 ab( rs=0.288, P=0.001) and N( rs=0.296, P=0.001) were positively correlated with sampling time. The Ct values of ORF1 ab in pharyngeal swab and sputum were 29.19±4.85 and 28.74±6.40, the Ct values of N in pharyngeal swab and sputum were 29.61±4.60 and 29.22±6.10, both without significant differences between different samples( P>0.05). Conclusion Sampling time has a great influence on the Ct values of ORF1 ab and N of SARS-CoV-2.

关 键 词：新型冠状病毒肺炎 严重急性呼吸综合征冠状病毒2型 核酸检测 靶标基因 

分 类 号：R563.1[医药卫生—呼吸系统]