玉米须总黄酮对胰岛素抵抗合并高尿酸血症大鼠模型相关代谢指标的影响  

Effects of total flavonoids in stigmata maydis on related metabolism indexes of rat models of insulin resistance complicated with hyperuricemia

作　　者：刘孟渊 冯学轩 黎莉斯 LIU Mengyuan;FENG Xuexuan;LI Lisi(The Affiliated TCM Hospital of Guangzhou Medical University,Guangzhou 510130,Guangdong,China;Guangdong Medical Laboratory Animal Center,Foshan 528248,Guangdong,China)

机构地区：[1]广州医科大学附属中医医院,广东广州510130 [2]广东省医学实验动物中心,广东佛山528248

出　　处：《上海中医药杂志》2020年第12期59-64,共6页Shanghai Journal of Traditional Chinese Medicine

基　　金：广东省广州市科技创新委员会科学(技术)研究专项一般项目(201607010303)。

摘　　要：目的观察玉米须总黄酮(TFSM)对胰岛素抵抗合并高尿酸血症(IR-HUA)模型大鼠血清尿酸(SUA)等相关代谢指标及胰岛素抵抗(IR)状态的影响。方法选用60只雄性SD大鼠,按体质量随机分为正常对照组、IR-HUA模型组(简称IR-HUA组)、吡格列酮阳性对照1组(简称吡格列酮组)、苯溴马隆阳性对照2组(简称苯溴马隆组)、TFSM高剂量组(简称TFSM.H组)和TFSM低剂量组(简称TFSM.L组)。正常对照组喂饲普通颗粒饲料和正常饮用水,其余各组每天喂饲高热能饲料及10%果糖水,连续8周;第7周始(d43),各给药组灌胃相应药液,正常对照组、IR-HUA组灌胃2%吐温80 CMC-Na溶媒,每日1次,连续15 d;给药3~4 h后,正常对照组灌胃0.5%CMC-Na溶液,其余各组灌胃腺嘌呤溶液(150 g/kg体质量),每日1次,连续15 d。结果与正常对照组相比,IR-HUA组的Lee’s指数及尿酸排泄分数(FEUA)增加;同时,IR-HUA组的空腹血糖(FPG)及HOMA-IR指数升高,空腹胰岛素(FINS)略有升高。与IR-HUA组相比,苯溴马隆组体长增加,Lee’s指数降低;苯溴马隆组、TFSM.H组、TFSM.L组的SUA及UUA浓度略有降低而排尿体积略有增加,吡格列酮组、苯溴马隆组、TFSM.H组、TFSM.L组的尿酸排泄总量均略有下降,但吡格列酮组、苯溴马隆组、TFSM.L组的FEUA均略有增加;同时,吡格列酮组、苯溴马隆组、TFSM.H组的FPG、FINS、HOMA-IR指数及TFSM.L组的FINS均略有降低。此外,苯溴马隆组、TFSM.H组的SUA水平与FEUA具有相关性。结论 IR-HUA模型大鼠在SUA升高的同时伴随FPG、Lee’s指数及HOMA-IR指数升高,表明制备的IR-HUA模型是成功的;TFSM、吡格列酮、苯溴马隆均显示出一定的促进模型大鼠肾脏排泄尿酸、降低SUA水平以及改善IR状态的作用趋势;SUA水平与肾脏尿酸排泄能力密切相关。Objective To observe the effect of total flavonoids in stigmata maydis(TFSM) on the concentration of serum uric acid(SUA) and other related metabolic indexes as well as insulin resistance(IR) state in rat models of insulin resistance complicated with hyperuricemia(IR-HUA rat models). Methods 60 male SD rats were randomly divided into the normal control group(Control group),the IR-HUA model group(IR-HUA group),the pioglitazone positive control group(Pioglitazone group),the benzbromarone positive control group(Benzbromarone group),the TFSM high dose group(TFSM.H group) and the TFSM low dose group(TFSM.L group). The Control group was fed with the ordinary feed and drinking water. The other groups were fed with high energy feed and drinking 10% fructose water for 8 weeks. At the beginning of the 7 th week(d43),the Control group and the IR-HUA group were given intragastric administration of 2% Tween 80 CMC-Na, and the other groups were given the corresponding medicine solution once a day for 15 days;3-4 h after intragastric administration,the Control group was given intragastric administration of 0.5% CMC-Na solution,the other groups were given adenine solution( 150 mg/kg body mass) once a day for15 days. Results Compared with the Control group,in the IRHUA group,the Lee’s index,the fraction excretion of uric acid( FEUA),the fasting plasma glucose( FPG) and the HOMA-IR index increased,and the fasting insulin( FINS) increased slightly.Compared with the IR-HUA group,the body length of the Benzbromarone group increased and the Lee’s index decreased;the concentrations of SUA and the urine uric acid( UUA) in the Benzbromarone group,TFSM.H group and TFSM.L group decreased slightly,while the urination volume increased slightly. The total uric acid excretion decreased slightly in the Pioglitazone group,Benzbromarone group,TFSM.H group and TFSM.L group,but FEUA increased slightly in the Pioglitazone group,Bebzbrommarone group and TFSM. L group. And the FPG,FINS,HOMA-IR index of the Pioglitazone group,Benzbromarone group,TF

关 键 词：玉米须总黄酮 胰岛素抵抗 高尿酸血症 代谢综合征 胰岛素抵抗-高尿酸血症(IR-HUA)模型 大鼠 

分 类 号：R285.5[医药卫生—中药学]