MicroRNA-31对HIV感染者CD4^+T细胞CD69表达的影响及机制  

Effect and mechanism of microRNA-31 on CD69 expression on CD4^+T cells of HIV-infected patients

作　　者：吕俊楠 陈亚利[1] 宋成博 傅雅静[1] 姜拥军[1] 张子宁[1] LÜJun-nan;CHEN Ya-li;SONG Cheng-bo;FU Ya-jing;JIANG Yong-jun;ZHANG Zi-ning(NHC Key Laboratory of AIDS Immunology,National Clinical Research Center for Laboratory Medicine,AIDS Institute of the First Affiliated Hospital of China Medical University,Shenyang 110001,China)

机构地区：[1]国家卫生健康委员会艾滋病免疫学重点实验室、国家医学检验临床医学研究中心、中国医科大学附属第一医院艾滋病研究所,沈阳110001

出　　处：《传染病信息》2020年第6期504-508,共5页Infectious Disease Information

基　　金：国家“十二五”科技重大专项(2015ZX10004801-002);国家自然科学基金(81371884)。

摘　　要：目的探讨微小RNA(microRNA,miR)-31对HIV感染者CD4^+T细胞表面CD69表达的影响及作用机制。方法通过流式细胞仪分析健康对照与HIV感染者的CD4^+T细胞活化后CD69表达情况。在健康人外周血单个核细胞中转染antagomir-miR-31抑制miR-31表达,观察CD4^+T细胞活化后CD69表达情况。采用生物信息学方法预测miR-31靶基因及相关通路,并在293T细胞系中过表达和抑制miR-31,通过实时荧光定量PCR检测相关靶基因的表达。结果与健康对照相比,HIV感染者CD4^+T细胞在抗-CD3/CD28抗体刺激活化后CD69表达更低。抑制miR-31表达后,CD4^+T细胞活化时CD69的表达与对照相比显著下降。miR-31低表达后T细胞活化信号通路KSR2和DUSP7表达升高。结论miR-31可以有效调节CD4^+T细胞表面CD69的表达,对HIV感染者早期免疫活化起重要作用。Objective To explore the effect and mechanism of microRNA-31(miR-31)on CD69 expression on CD4^+T cells in HIV-infected patients.Methods CD69 expression on stimulated CD4^+T cells from healthy controls and HIV-infected patients was analyzed by flow cytometry.When peripheral blood mononuclear cells from healthy controls were transfected with antagomir-miR-31 to inhibit miR-31 expression,CD69 expression on CD4^+T cells after stimulation was observed.The target gene of miR-31 and related pathway were predicted by bioinformatics method and the expression of related target gene was verified by overexpression and inhibition of miR-31 in 293 T cell line based on quantitative real-time PCR.Results Compared with healthy controls,CD69 expression on CD4^+T cells from HIV-infected patients was significantly lower after anti-CD3/CD28 antibody stimulation.CD69 expression on CD4^+T cells was significantly decreased when miR-31 expression was inhibited,compared with controls.The expression of KSR2 and DUSP7 in T cell activation signal pathway was increased upon the low expression of miR-31.Conclusions miR-31 can effectively regulate CD69 expression on surface of CD4^+T cells and plays an important role in early immune activation in HIV-infected individuals.

关 键 词：HIV miR-31 CD69 CD4^+T细胞 早期免疫活化 KSR2 DUSP7 

分 类 号：R512.91[医药卫生—内科学] R361.3[医药卫生—临床医学]