化学发光法与胶体金法检测SARS-CoV-2抗体阳性率比较  

Comparison of chemiluminescence method and colloidal gold method for detecting SARS-CoV-2 antibody positive rate

作　　者：张鹏[1] 周文杰[2] 李志海 卓海燕 ZHANG Peng;ZHOU Wen-jie;LI Zhi-hai;ZHUO Hai-yan(Department of Respiratory and Critical Care Medicine,General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学总医院呼吸与危重症医学科,银川750004 [2]宁夏医科大学总医院ICU,银川750004 [3]襄阳市襄州区人民医院普通内科,441000 [4]襄阳市襄阳州区第二人民医院内科,441000

出　　处：《传染病信息》2020年第6期533-535,共3页Infectious Disease Information

基　　金：宁夏医科大学总医院新冠肺炎疫情防控应急项目(XE202010,XE202018);宁夏医科大学校级科研项目(XY2017132);宁夏青年科技人才托举工程项目(TJGC20190096,TJGC20190099)。

摘　　要：目的探讨化学发光法和胶体金法检测SARS-CoV-2抗体在2019冠状病毒病(coronavirus disease 2019,COVID-19)诊断中的应用价值。方法收集51例COVID-19确诊患者和41例非COVID-19患者的血清,用化学发光法检测非COVID-19组血清中SARS-CoV-2抗体IgM、IgG及联合检测(IgM或IgG)的阳性率;用化学发光法和胶体金法检测COVID-19组血清中SARS-CoV-2抗体IgM、IgG及联合检测(IgM或IgG)的阳性率,比较2种方法的一致性。结果COVID-19组平均发病时间为(16.9±5.4)d。其中22例IgM阳性,阳性率为43.1%;47例IgG阳性,阳性率为92.2%;49例联合检测(IgM或IgG)阳性,阳性率为96.1%。非COVID-19组中,1例IgM阳性,阳性率为2.4%;0例IgG阳性,阳性率为0;1例联合检测(IgM或IgG)阳性,阳性率为2.4%。在COVID-19组中,化学发光法与胶体金法检测SARS-CoV-2抗体联合检测(IgM或IgG)的阳性率分别为96.1%和68.6%,化学发光法优于胶体金法(P<0.05)。结论SARS-CoV-2抗体的检测对COVID-19诊断有重要的意义,且化学发光法检出率优于胶体金法。Objective To explore the application of SARS-CoV-2 antibodies in diagnosis of coronavirus disease 2019(COVID-19)by chemiluminescence method and colloidal gold method.Methods The sera were selected from 51 patients with confirmed COVID-19 and 41 patients without COVID-19.The positive rates of antibody IgM,IgG and combined detection(IgM or IgG)in serum of non-COVID-19 group were detected by chemiluminescence method,and the positive rate of antibody IgM,IgG and combined detection(IgM or IgG)in patients of COVID-19 group was detected by chemiluminescence method and colloidal gold method,then the consistency of 2 methods was compared.Results In the COVID-19 group,the average onset time of patients was(16.9±5.4)days.There were 22 cases positive for IgM,47 cases positive for IgG and 49 cases positive for combined dection,with the positive rates of 43.1%,92.2%and 96.1%.In the non-COVID-19 group,there was 1 case positive for IgM,0 case positive for IgG and 3 cases positive for combined dection,with the positive rate of 2.4%,0 and 2.4%.Among the 51 COVID-19 cases,the positive rates of SARS-CoV-2 antibody combined dection by the chemiluminescence method and colloidal gold method were 96.1%and 68.6%.The chemiluminescence method was better than the colloidal gold method(P<0.05).Conclusions The detection of antibodies against SARS-CoV-2 is of great significance for the diagnosis of COVID-19,and the detection rate of chemiluminescence method is superior to that of colloidal gold method.

关 键 词：新型冠状病毒 化学发光法 胶体金法 抗体 

分 类 号：R563.1[医药卫生—呼吸系统]