两亲性壳聚糖自组装纳米微球的制备及抗真菌性能研究  

Preparation and Antifungal Properties of Self-assembledAmphiphilic Chitosan Nanocapsules

作　　者：何祖宇 谢江辉[1] 李普旺 屈云慧 杨子明 于丽娟 王超 刘运浩 姚全胜[1] 周闯 HE Zuyu;XIE Jianghui;LI Puwang;QU Yunhui;YANG Ziming;YU Lijuan;WANG Chao;LIU Yunhao;YAO Quansheng;ZHOU Chuang(Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products,South Subtropical Crop Research Institute of China Academy of Tropical Agricultural Sciences,Zhanjiang 524091,China;Agricultural Product Processing Research Institute of Yunnan Academy of Agricultural Sciences,Kunming 650000,China)

机构地区：[1]中国热带农业科学院南亚热带作物研究所,海南省热带园艺产品采后生理与保鲜重点实验室,湛江524091 [2]云南省农业科学院农产品加工研究所,昆明650000

出　　处：《材料导报》2020年第S02期501-506,共6页Materials Reports

基　　金：海南省自然科学基金(219QN294);广东省基础与应用基础研究基金(2019A1515010714);云南省李普旺专家工作站(202005AF150007);中国热带农业科学院基本科研业务费专项项目(1630122017009,1630122020004);广东省现代农业产业技术体系创新团队建设专项资金(2019KJ140);广东省农业科技创新体系管理项目(0835-190Z22404211C1);湛江市科技项目资助(2018A02015)。

摘　　要：通过两步法反应将桐油酸(EA)和N-乙酰-L-半胱氨酸(NAC)接枝壳聚糖(CS)制备两亲性壳聚糖(CS-g-EA-NAC),并通过红外光谱、核磁氢谱对其结构进行表征分析。然后采用超声波自组装法制备了负载多杀菌素(Spinosad,SSD)的载药纳米粒子(SSD@CS-g-EA-NAC)。最后通过生长速度法测定了SSD@CS-g-EA-NAC和SSD对尖孢镰刀菌的抗菌性能。结果表明,载药纳米粒子呈球形,粒径分布均匀,平均粒径约为520 nm,同时载药纳米粒子具有良好的缓释性能和pH响应性能。抑菌实验显示,载药纳米粒子(SSD@CS-g-EA-NAC)和SSD对尖孢镰刀菌的半数有效浓度(EC 50)分别是29.05μg/mL和42.05μg/mL,这表明SSD@CS-g-EA-NAC毒力高于游离的SSD,纳米粒子(CS-g-EA-NAC)有助于提高SSD的毒力性能。该研究可为壳聚糖基载药体系的构建提供理论依据。In this paper,eleostearic acid(EA)and N-acetyl-L-cysteine(NAC)were grafted to the chain of chitosan(CS)to prepare an amphiphilic chitosan derivative(CS-g-EA-NAC),and the chemical structure of CS-g-EA-NAC was characterized by Fourier transform infrared(FT-IR)and 1H nuclear magnetic resonance(1H NMR).Then,CS-g-EA-NAC based nanoparticles were prepared by ultrasonic self-assembly method,and the spinosad(SSD)loaded nanocapsules(SSD@CS-g-EA-NAC)were spherical in shape with a uniform particle size distribution,and the average particle size was about 520 nm.In vitro release revealed that the SSD@CS-g-EA-NAC nanocapsules exhibited a sustained and pH-responsive drug release property.Finally,the antifungal properties of SSD@CS-g-EA-NAC and SSD against Fusarium oxysporum were determined by the growth rate method.The results showed that the half effective concentration(EC 50)of SSD@CS-g-EA-NAC and SSD against Fusarium oxysporum was were 29.05μg/mL and 42.05μg/mL,respectively,indicating that the virulence of SSD@CS-g-EA-NAC is higher than that of free SSD,and the conjugate CS-g-EA-NAC is conducive to improve the virulence of SSD.This research can provide a theoretical basis for the construction of chitosan-based drug delivery system.

关 键 词：壳聚糖 自组装 多杀菌素 纳米微球 抗菌 

分 类 号：TQ314.1[化学工程—高聚物工业]